Establishment of Hamster-and Human-PRNP Transgenic Mice  

作　　者：GONG Han Shi TIAN Chan ZHANG Bao Yun WANG Zhao Yun XIE Wu Ling JING Yuan Yuan GAO Chen JIANG Hui Ying SHI Qi LIU Yong DONG Xiao Ping 

机构地区：[1]School of Medicine, Xi＇an Jiao Tong University, Xi＇an 710061, Shaanxi, China [2]State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

出　　处：《Biomedical and Environmental Sciences》2011年第6期608-616,共9页生物医学与环境科学（英文版）

基　　金：supported by Chinese National Natural Science Foundation Grants 30771914 and 30800975;Institution Technique R&D Grant (2008EG150300);National Basic Research Program of China (973 Program) (2007CB310505);China Mega-Project for Infectious Disease (2009ZX10004-101);the SKLID Development Grant (2008SKLID102 and 2008SKLID202)

摘　　要：Objective To create transgenic mice expressing hamster- and human-PRNP as a model tor understanding the physiological function and pathology of prion protein （PrP）, as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies （TSEs）. Methods Hamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin （H ＆ E） staining and immunohistochemical （IHC） methods. Results Integrated PRNP copy number in various mouse lines was 53 （Tg-haPrP1）, 18 （Tg-huPrP1）, 3 （Tg-huPrP2）, and 16 （Tg-huPrP5）, respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs. Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.Objective To create transgenic mice expressing hamster- and human-PRNP as a model tor understanding the physiological function and pathology of prion protein （PrP）, as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies （TSEs）. Methods Hamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin （H ＆ E） staining and immunohistochemical （IHC） methods. Results Integrated PRNP copy number in various mouse lines was 53 （Tg-haPrP1）, 18 （Tg-huPrP1）, 3 （Tg-huPrP2）, and 16 （Tg-huPrP5）, respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs. Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.

关 键 词：PRP PRNP Transgenic mice Copy number 

分 类 号：S852.659.7[农业科学—基础兽医学] Q78[农业科学—兽医学]