生防菌哈茨木霉T4对黄瓜根围土壤细菌群落的影响  

Effects of the Introduction of Biocontrol Agent Trichoderma harzianum T4 on the Bacterial Community in Cucumber Rhizosphere

作　　者：尹丹韩[1] 高观朋[1] 夏飞[1] 王伟[1] 

机构地区：[1]华东理工大学生物工程学院/生物反应器工程国家重点实验室,上海200237

出　　处：《中国农业科学》2012年第2期246-254,共9页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30871664);国家重点实验室专项经费(2060204)

摘　　要：【目的】评价生防菌哈茨木霉(Trichoderma harzianum)T4对根围土壤细菌群落的影响,为生防菌田间应用的生态安全提供依据。【方法】结合DGGE技术与T-RFLP方法,综合考察引入生防菌哈茨木霉T4后黄瓜根围土壤细菌群落变化。【结果】在引入生防菌的14—56 d内(黄瓜整个生育期),木霉对根围土壤细菌群落可产生显著影响,并持续一段的时间,随后逐渐减弱。表现为对蓝细菌、β-变形细菌、葡萄球菌、伯克霍尔德菌、沙雷氏菌、酸杆菌和一些不可培养细菌的有效抑制作用,对芽孢杆菌、土壤杆菌、芽单胞菌的明显促进作用。但到70 d时,即收获后2周,检测不到木霉对细菌群落影响。【结论】DGGE和T-RFLP序列分析技术相结合研究生防菌对土壤细菌群落的影响,既能全面描述土壤细菌群落结构变化,还可以初步定性描述变化细菌的种类信息。生防菌哈茨木霉T4的引入对土壤细菌群落产生短期影响,不会长期对土壤细菌群落稳定构成威胁。【Objective】The objective of this study is to analysis the impact of T.harzianum T4 introduction on the indigenous bacterial community of rhizosphere soil,and to provide objective biosafety evaluations for the field application of biocontrol agents.【Method】 Comprised molecular tools DGGE and T-RFLP were used in this study to assess the modifications of the bacterial community in cucumber rhizosphere after inoculation with T.harzianum T4.【Result】 Introduction of T.harzianum T4 in the soil resulted in significant variations of the bacterial community structures from 14 to 56 days post-inoculation.During this period the impact of T4 was sustainable and weakened gradually.T.harzianum T4 could restrain effectively Cyanobacterium,Beta-proteobacterium,Staphylococcus,Burkholderia,Serratia,Acidobacterium and some kinds of soil uncultured bacterium,while it improved the growth of Bacillus,Agrobacterium and Gemmatimonadetes observably.On 70th day after T.harzianum T4 introduced,as 2 weeks after harvest,there was no prominent alteration of bacterial community due to inoculation with T4.【Conclusion】 Tracking of the modifications of the indigenous bacterial community by 16S rDNA DGGE and T-RFLP analysis not only described comprehensively the variations of the structure of soil bacterial community,but also showed the preliminary and qualitative information of bacterial community change.In general,the application of T.harzianum T4 exerted only transient impact on the bacterial community of cucumber rhizosphere in systematic field investigation,but the perturbation was unable to imperil the bacterial community stability in the long-term.

关 键 词：哈茨木霉T4 土壤细菌群落 T-RFLP DGGE 

分 类 号：S154.3[农业科学—土壤学] S476.1[农业科学—农业基础科学]