TGF-β1对人肝癌细胞株HepG2的FGFR4表达的影响及意义  

Effect of TGF-β1 on FGFR4 expression in HepG2 cells

作　　者：江松敏[1] 蔡琳[1] 朱婷婷[1] 施立华[1] 金芩[1] 夏清海[1] 

机构地区：[1]温州医学院药学院,325035

出　　处：《浙江医学》2011年第12期1787-1789,1814,共4页Zhejiang Medical Journal

基　　金：基金项目：浙江省教育厅科研项目（Y200907097）;温州市科技计划项目（2（）f）90257）

摘　　要：目的 通过观察转化生长因子β1（TGF-β1）对肝癌细胞株HepG2的成纤维细胞生长因子受体4（FGFR4）表达的影响,探讨其抑制肝癌细胞增殖的作用机制.方法 采用Western blot方法检测不同诱导时间（0、0.5、2、6、12、16、24h）、不同TGF-β1浓度（0、0.01、0.1、1、5ng/ml）对HepG2的FGFR4表达的影响.同时观察TGF-β1与不同FGFs联合应用对肝癌细胞增殖的影响.结果 在相同TGF-β1浓度下,FGFR4的表达随时间的延长而增强（P＜0.01）,HepG2细胞数随时间的延长而减少（P＜0.01）.在相同诱导时间下,FGFR4的表达随TGF-β1浓度的增大而增强（P＜0.01）,HepG2细胞数随TGF-β1浓度增加而减少（P＜0.01）.TGF-β1对FGF1、FGF2、FGF7效应均有显著增强作用,可使相应HepG2细胞数显著增加（P＜0.01）;对FGF21效应具有显著抑制作用,可使相应HepG2细胞数显著减少（P＜0.01）.结论 TGF-β1可诱导肝癌细胞株HepG2的FGFR4表达,与FGFs合用可影响肝癌细胞的增殖.Objective To investigate the effect of TGF-β1 on expression of fibroblast growth factor receptor 4 （FGFR4） in hepatocellular carcinoma HepG2 cells. Methods The expression of FGFR4 in HepG2 cells were detected by Western blot with the different induction time （0, 0.5h, 2h, 6h, 12h, 16h, 24h） and different TGF-131 dosage （0ng/ml, 0.01ng/ml, 0.1ng/ml, lng/ml, 5ng/ml ）. The effects on proliferation of HepG2 cells induced by combination of TGF-β1 with different FGFs were also observed. Results With the same concentration of TGF-β1, FGFR4 expression was increased by time extension （P〈0.01）; while the number of HepG2 cells was decreased accordingly （P〈0.01）. With the same time, FGFR4 expression was enhanced by increasing concentration of TGF-131 （P〈0.01）; while the number of HepG2 cells was decreased accordingly （P〈0.01）. The combination of TGF-β1 with FGF1, 2, 7 promoted HepG2 proliferation （P〈0.01）, where with FGF21 the cell proliferation was inhibited （P〈 0.01 ）. Conclusion TGF-61 can induce FGFR4 expression and influence proliferation by combination with FGFs in human hepa- tocellular carcinoma HepG2 cells.

关 键 词：转化生长因子Β1 成纤维细胞生长因子受体4 HEPG2细胞 

分 类 号：R977.9[医药卫生—药品]