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作 者:汤日圣[1] 童红玉[1] 黄益洪[1] 叶晓青[1]
机构地区:[1]江苏省农业科学院,江苏省农业生物学重点实验室,江苏南京210014
出 处:《江苏农业学报》2011年第6期1210-1215,共6页Jiangsu Journal of Agricultural Sciences
摘 要:为了探讨高温危害冷季型草坪草的生理生化机制及外源ABA和4PU-30的调节作用,以草地早熟禾(Poa pratensts L.cv.Total Eclipse)的幼苗为材料,研究了高温胁迫下其植株中有关生理指标的变化及外源脱落酸(ABA)和4PU-30的调节效应。结果表明:在(42.0±0.5)℃高温胁迫下,幼苗质膜透性和丙二醛含量明显增加,超氧化物歧化酶(SOD)活性和可溶性蛋白质及还原型谷胱甘肽(GSH)含量明显降低;而可溶性糖、脯氨酸和抗坏血酸(AsA)含量在高温胁迫初期都有所增加,其后则都明显降低。高温胁迫前用10 mg/L ABA和10 mg/L 4PU-30处理,对高温胁迫下冷季型草坪草生理指标的变化有明显的调节作用,能有效减缓高温胁迫下质膜透性和丙二醛含量的增加,有效减缓高温胁迫下SOD活性和可溶性蛋白质、GSH、可溶性糖、脯氨酸和AsA含量的降低。In order to explore the physiological mechanism of high temperature injury to cool-season turfgrass and regulations by exogenous plant growth substance,the effect of high temperature [(42.0±0.5)℃] on some physiological indexes in the seedlings of Total Eclipse(Poa pratensts L.)and the regulations of ABA and 4PU-30 were studied.The results showed that electrolyte leakage and malondialdehyde(MDA) content in seedlings increased significantly under high temperature stress,while the activities of superoxide dismutase(SOD) and the contents of soluble protein and glutathione(GSH)decreased significantly.The contents of soluble sugar,proline and ascorbic acid(AsA)increased slightly on day 2 under high temperature stress,but decreased significantly afterwards.The exogenous ABA(10 mg/L)and 4PU-30(10 mg/L) could slow down effectively the increase of electrolyte leakage and MDA content in seedlings during high temperature stress and the decrease of SOD activities and the contents of AsA,GSH,proline,soluble protein and soluble sugar in seedlings during the high temperature stress.
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