骨髓基质细胞与生物活性玻璃陶瓷和聚乳酸生物相容性的实验研究  

BIOCOMPATIBILITY STUDIES ON BIOACTIVE GLASS CERAMICS AND POLYLACTIC ACID COMBINED WITH CULTURED BONE MARROW STROMAL CELLS IN VITRO

作　　者：金丹[1] 裴国献[1] 王前[1] 

机构地区：[1]第一军医大学附属南方医院创伤骨科,广州510515

出　　处：《中国修复重建外科杂志》2000年第1期39-43,共5页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的　探讨骨髓基质细胞与生物活性玻璃陶瓷(BGC) 和聚乳酸(PLA) 的生物相容性,为骨组织工程中生物材料的选择提供依据。方法　将骨髓基质细胞与BGC、PLA 复合体外培养,进行形态学观察、细胞增殖、蛋白质含量及酶学测定。结果　骨髓基质细胞能在BGC、PLA 上贴附、繁殖,其生长及功能不受影响,并且BGC具有一定的促细胞增殖作用。结论　BGC、PLA 具有良好的细胞相容性。Objective To study the biocompatibility on bioactive glass ceramics (BGC) and polylactic acid (PLA) combined with cultured bone marrow stromal cells (BMSCs) in bone tissue engineering. Methods BMSCs were cultured combined with BGC and PLA in vitro , and the morphological characters, cell proliferation, protein content, and alkaline phosphatase activity were detected. Results BMSCs could be attached to and extended on both BGC and PLA, and normally grown, proliferated, had active function. BGC could promote cell proliferation. Conclusion The results show that both BGC and PLA have good biocompatibility with BMSCs, they can be used as biomaterials for cell transplantation in tissue engineering.

关 键 词：骨髓基质细胞 生物材料 生物相容性 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]