三叉神经节内降钙素基因相关肽的分布及其在伤害性刺激条件下的变化  

DISTRIBUTION OF CGRP NEURONS IN THE TRIGEMINAL AND CHANGES OF CGRP-LIKE IMMUNOREACTIVITY RESPONDING TO NOXIOUS STIMULATION

作　　者：蒋吉英[1] 窦寰宇[1] 高培福[1] 朱世杰[1] 李光宗[1] 杨培花 

机构地区：[1]潍坊医学院解剖学教研室 [2]潍坊交通医院,潍坊261042

出　　处：《解剖学杂志》2000年第1期52-55,共4页Chinese Journal of Anatomy

摘　　要：目的：研究三叉神经节内降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎ ｇｅｎｅｒｅｌａｔｅｄ ｐｅｐｔｉｄｅ，ＣＧＲＰ）的分布及其在伤害性刺激条件下的变化。方法：将 １０％福尔马林 １５０～２００ ｕｌ注入 ８只 Ｗｉｓｔａｒ大鼠面口部皮下，１／２、２、１２ ｈ取材，用 ＡＢＣ法行免疫组化染色，图像分析仪测量阳性细胞的积分光密度。结果：（１）ＣＧＲＰ阳性细胞以神经节的外周为著，多为中等细胞（８９％）。（２）染色深浅与细胞的大小无关。（３）面口部伤害性刺激条件下，节细胞内ＣＧＲＰ阳性产物的积分光密度在 Ｆ１／２ ｈ组较对照组下降（Ｐ＜０． ０５）；Ｆ２ｈ组较对照组升高（Ｐ＜０． ００１）；Ｆ１２ ｈ组较对照组无明显差异（Ｐ＞０．０５）。结论：（１）福尔马林刺激后，三叉神经节细胞中ＣＧＲＰ的积分光密度发生时间相关性变化。（２）在三叉神经系统，ＣＧＲＰ参与伤害性刺激的传递。To study the distribution of CGRP neurons in the rat trigeminal ganglion and changes of CGRP-like immunoreactivity responding to ora-facial noxious stiumulation. Methods: Using the immunohistochemistry ABC technique and computer assisted image processing system, the changes of CGRP-like immunoreactivity(CGRP-li) in rat trigeminal ganglion were investigated semi-quantatively at different points (1/2h ; 2h; 12h) after injection of 150-200 ul 10% formalin under ora-facial skin. Results: (1 ) In rat trigeminal ganglion, there were more CGRP neurons near periphery than central, and most (89% ) CGRP neurons were medium--sized. (2)The dinsity of CGRP immunoreactive staining in the trigeminal ganglion was not correlated with cell size. (3) The optid of CGRP-h in rat trigeminal ganglion after formalin injection was different depending on the time of animal surviving. F1/2h group was lower, F2h group higher than and F12h group similar to that of the control group.

关 键 词：三叉神经 三叉神经节 CGRP 分布 伤害性刺激 

分 类 号：R338[医药卫生—人体生理学]