血流生理脉动切应力与内皮细胞一氧化氮产生的关系  

EFFECTS OF PHYSIOLOGIC PULSATILE SHEAR STRESS OF FLOW ON NITRIC OXIDE SYNTHESIS BY CULTURED ENDOTHELIAL CELLS

作　　者：艾珣 王克强[1] 赵子琴[1] 江国伟 贾剑国 

机构地区：[1]上海医科大学华山医院手外科

出　　处：《解剖学杂志》2000年第1期11-14,共4页Chinese Journal of Anatomy

基　　金：卫生部科研基金资助项目!(No. 94-1-149)

摘　　要：目的：研究生理脉动切应力对血管内皮细胞一氧化氮产生的影响。材料与方法：利用本室已建立的平行板模拟血流循环装置，在模拟生理脉动切应力（１９． ２９，５６． ２５ ｄｙｎｅｓ／ｃｍ２）分别作用于培养人脐静脉内皮细胞 ０，１，２，４，６，１０，２４ ｈ后，用一氧化氮微量测定法测定循环液中一氧化氮的含量。结果：在两组模拟生理脉动切应力的作用下，于２ｈ时一氧化氮释放出现一峰值，之后逐渐下降，并且内皮细胞释放一氧化氮的量随切应力的增大而增加。结论：生理脉动切应力对血管内皮细胞一氧化氮的产生具有调节作用，可能对血管动脉粥样硬化的形成与发展中，临床手术前后发生的血管痉挛与血栓形成等增多有重要影响。To study the effect of physiologic pulsatile shear stress of low on nitric oxide synthesis by cultured endothelial cells (EC). Methods: The parallel plate flow-loading apparatus was used to imitate the physilogic pulsatile shear stress (19. 29 and 56. 25 dynes/cm2) and the concentrations of nitric oxide (NO) in flow were measured among test groups and different time points. Results: NO production by EC were increased under the pulsatile shear stress condition, the releasing of NO reached its peak on 2 h, and then it declined gradually. The production of NO by EC increased with the increase of the pulsatile shear stress. Conclusion: This results indicate that physiologic pulsatile shear stress modulate the production of NO by EC.

关 键 词：内皮细胞 一氧化氮 生理脉动切应力 

分 类 号：R331[医药卫生—人体生理学]