Effect of initial substrate and biomass concentrations on anaerobic fermentative hydrogen production in batch reactors  

作　　者：昌盛 李建政 刘枫 王淑静 

机构地区：[1]State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology

出　　处：《Journal of Harbin Institute of Technology(New Series)》2011年第6期11-15,共5页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by the State Key Laboratory of Urban Water Resource and Environment of Harbin Institute of Technology(Grant No.2010DX06);the National High Technology Research and Development Program of China(Grant No.2006AA05Z109);the Harbin Science and Technology Bureau(Grant No.2009RFXXS004)

摘　　要：The effects of initial substrate (5-60 g /L) and biomass concentration (0.5-3 g /L) on fermentative hydrogen production by mixed cultures were investigated in batch tests using glucose as substrate.The experimental results showed that the hydrogen production increases as the initial substrate concentration increases from 0 to 25 g /L.It indicated that the shift in the metabolic pathway or in the composition of the bacterial flora occurs.The maximum hydrogen yield of 1.78 mol /mol-glucose is obtained at the substrate concentration of 15 g /L.This study also shows that initial biomass concentration affects the hydrogen yield as the cumulative hydrogen production has been increased with the increase of initial cell concentration up to 1.5 g /L and reached the highest level.The maximum hydrogen yield is obtained at the cell concentration of 1.5 g /L.It indicated that the optimum biomass /substrate ratio,maximizing the hydrogen yield and the hydrogen production rate,is determined to be 0.1 g biomass /g glucose.The effects of initial substrate （5 -60 g/L） and biomass concentration （0. 5 -3 g/L） on fermentative hydrogen production by mixed cukures were investigated in batch tests using glucose as substrate. The experimental results showed that the hydrogen production increases as the initial substrate concentration increases from 0 to 25 g/L. It indicated that the shift in the metabolic pathway or in the composition of the bacterial flora occurs. The maximum hydrogen yield of 1.78 mol/mol-glucose is obtained at the substrate concentration of 15 g/L. This study also shows that initial biomass concentration affects the hydrogen yield as the cumulative hydrogen production has been increased with the increase of initial cell concentration up to 1.5 g/L and reached the highest level. The maximum hydrogen yield is obtained at the cell concentration of 1.5 g/L. It indicated that the optimum biomass/substrate ratio, maximizing the hydrogen yield and the hydrogen production rate, is determined to be O. 1 g biomass/g glucose.

关 键 词：fermentative hydrogen production hydrogen yield substrate concentration biomass concentration modified Gompertz model 

分 类 号：X703[环境科学与工程—环境工程]