装载于PLGA中的5-氟尿嘧啶缓释微球的制备及其体外释放的研究  

Study on Preparation and in Vitro Release of 5-Fluorouracil Loaded in PLGA Sustained-Release Microspheres

作　　者：赵仲[1] 孟乐乐[2] 陈霓祥[2] 胡振华[2] 陈思源[2] 王薪懿[1] 林清[2] 苏靖[1] 

机构地区：[1]上海交通大学,上海200240 [2]上海交通大学医学院附属新华医院,上海200092

出　　处：《现代生物医学进展》2011年第24期4801-4803,共3页Progress in Modern Biomedicine

基　　金：上海交通大学医工结合基金(YG2010MS11);国家自然科学基金(81001416)

摘　　要：目的:研究装载于不同分子量的PLGA中的5-氟尿嘧啶微球的制备方法及其在体外条件下的缓释行为。方法:以水包油包固复乳法将5-氟尿嘧啶包裹在高分子聚乳酸-聚羟基乙酸共聚物(PLGA)中,形成缓释微球,考察其大小,外观,包封率等理化性质,以紫外分光光度法为检测方法研究其体外释放行为。结果:经扫描电子显微镜观察,所制备的微球形态完整,大小较均匀。具有一定得包封率和载药量,体外释放研究表明其处方1和处方2的缓释时间为8天和23天。结论:以水包油包固复乳法制备的PLGA 5-氟尿嘧啶微球能够达到缓释的目的。Objective： To investigate the preparation method and in vitro release behavior of 5-Fluorouracil loaded in sustained-re-lease microspheres prepared by PLGA of different molecular weight.Methods： 5-Fluorouracil was microencapsulated into PLGA micro-spheres by solid-in-oil-in-water（S/O/W） multiple emulsion technique,then the sustained-release microspheres were obtained.The physical and chemical properties of the microspheres such as the particle size,appearance and encapsulation efficiency were detected.The micro-spheres＇ in vitro release behaviors were detected by the way of UV spectrophotometry.Results： The microspheres had complete shape,uniform particle size.They had certain encapsulation efficiency and loading capacity.The research of in vitro release showed that the formulation1 and formulation 2 can release 5-Fu 8 days and 23 days respectively.Conclusion： The microspheres prepared by the way of solid-in-oil-in-water（S/O/W） multiple emulsion technique can reach the target of sustained-release of 5-Fu.

关 键 词：PLGA 5-氟尿嘧啶 缓释微球 体外释放 

分 类 号：R979.1[医药卫生—药品] R918[医药卫生—药学]