应用花药培养提纯粳稻不育系XG111A和保持系XG111B  

Purification and Rejuvenation of CMS Lines XG111A and Maintainer Lines XG111B through Anther Culture

作　　者：胡婷婷[1] 刘超[1] 王健康[1] 丁成伟[1] 郭荣良[1] 吴玉玲[1] 徐家安[1] 王友霜[1] 

机构地区：[1]江苏徐淮地区徐州农业科学研究所,江苏徐州221121

出　　处：《西北农业学报》2011年第10期53-57,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：徐州市农业科学院科技创新基金(200804);江苏省科技支撑计划项目(BE2009301-1);国家科技支撑计划子课题(2006BAD01A01-7)

摘　　要：应用花药培养技术对粳稻BT型不育系XG111A和保持系XG111B进行提纯改良。结果表明,低温6～8℃预处理3～18 d,有助于花药愈伤诱导率的提高,尤以低温预处理8 d,愈伤诱导率最高;35～50 d诱导愈伤的分化率最高,XG111A和XG111B的分化率分别达到22.5%和30.2%。经花药培养后,不育系和保持系株系内整齐度均得到显著提高:不育系的不育花粉率为100%,原供体对照为99.58%。保持系的花培后代植株90%以上都具有原供体植株的典型特征,且后代的性状遗传稳定,另有部分株系优于原供体植株。Application of anther culture in purification and rejuvenation of CMS lines XG111A and maintainer lines XG111B in rice,the results showed that： it was contributed to the improvement of anther callus induction rate that pretreated for 3-18 d at low temperature of 6-8 ℃.Especially,callus induction rate was highest for 8 d at 6-8 ℃;Differentiation rate of callus induced between 35 d and 50 d was highest for XG111A and XG111B,22.5% and 30.2% respectively;sterile rate of pollen in CMS lines of anther culture was 100%;99.58% in the field.The uniformity in lines of CMS and maintainer had been significantly improved through anther culture.About 90% normal plants obtained by anther culture showed genetic stability and some plants are superior to donor plants.

关 键 词：粳稻 花药培养 提纯 不育系XG111A 保持系XG111B 

分 类 号：S511.22[农业科学—作物学]