肺成纤维细胞增殖与其合成胶原功能间的关系  

The relationship between proliferation and collagen formation of pulmonary fibroblasts

作　　者：陈晓玲[1] 陈祥银[2] 严仪昭 西品香[2] 

机构地区：[1]河北医科大学基础医学研究所病理生理研究室,石家庄050017 [2]中国协和医科大学基础医学研究所病理生理研究室

出　　处：《中华劳动卫生职业病杂志》2000年第1期39-41,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：美国中华医学基金会医学师资奖学金资助!项目 (第 941 1号 )

摘　　要：目的　探讨肺成纤维细胞 (FB)增殖与其合成胶原功能间的关系。方法　通过卟啉醇肉豆蔻酸乙酸乙酯 (PMA)和 1 (5 异喹啉基 ) 2 甲基哌嗪 (H 7)改变肺FB的增殖状态 ,观察肺FB数、细胞内DNA中3 H 胸腺嘧啶核苷 (3 H TdR)掺入量与细胞新合成蛋白质中3 H 脯氨酸 (3 H Pro)掺入量的关系。结果　 2 0nmol/L的PMA作用 3d时 ,肺FB数 [(12 .2± 2 .3)× 10 4 个 /孔 ]和细胞内DNA中3 H TdR掺入量 [(1.89± 0 .0 9)× 10 3 Bq]分别较对照组 [(7.5±1.1)× 10 4 个 /孔、(1.6 7± 0 .16 )×10 3 Bq]明显增多 ,差异有显著性 (P <0 .0 1) ;但细胞分泌的蛋白质中3 H Pro掺入量 [(6 87.5± 2 0 0 .5 )Bq/ 10 5cells]比对照组 [(1181± 147.5 )Bq/ 10 5cells]却明显减少 ,差异有显著性 (P <0 .0 1)。5 0 μmol/L的H 7作用 3d时 ,肺FB数 [(4.2± 0 .8)× 10 4 个 /孔 ]和细胞内3 H TdR掺入量 [(0 .72±0 .13)× 10 3 Bq]分别较对照组 [(7.5± 1.1)× 10 4 个 /孔、(1.12± 0 .0 8)× 10 3 Bq]明显减少 ,差异有显著性 (P <0 .0 1) ,但3 H Pro掺入量 [(170 5 .5± 2 5 1.5 )Bq/ 10 5cells]比对照组 [(1181± 147.5 )Bq/ 10 5cells]明显增多 ,差异有显著性 (P <0 .0 1)。结论　肺FB合成胶原蛋白的功能与其增殖状态有关。Objective To investigate the relationship between the proliferation and collagen accumulation of pulmonary fibroblasts. Methods Cultured pulmonary fibroblasts(FB) were stimulated by PMA(phorbol 12 myistate 13 acetate),an agonist of protein kinase C(PKC),and H 7[(1 5 isoquinolinyl) 2 methylpiperazine],the inhibitor of PKC,to alter the state of proliferation.The cell number of FB,the incorporation of 3H thymidine( 3H TdR) in DNA and the incorporation of 3H proline( 3H Pro) of new synthetic collagen were observed. Results 20 nmol/L PMA markedly increased the cell number [(12.2±2.3)× 10 4cells/well vs (7.5± 1.1) ×10 4cells/well, P < 0.01] and the incorporation of 3H TdR [(1.89± 0.09)×10 3 Bq vs (1.67±0.16)×10 3 Bq, P < 0.01] ,but decreased the incorporation of 3H Pro [(687.5± 200.5) Bq/10 5cells vs (1 181±147.5) Bq/10 5cells, P <0.01].On the other hand,50 μmol/L H 7 decreased the cell number[(4.2±0.8)×10 4 cells/well vs (7.5± 1.1) ×10 4 cells/well, P < 0.01] and the incoporation of 3H TdR [ (0.72± 0.13)×10 3 Bq vs (1.12±0.08)×10 3 Bq, P < 0.01], but increased the incorporation of 3H Pro [(1 705.5±251.5) Bq/10 5cells vs (1 181± 147.5) Bq/10 5cells, P < 0.01] . Conclusion The collagen synthesis is reversedly related to the proliferative state of pulmonary fibroblasts.

关 键 词：蛋白激酶C 肺成纤维细胞 增殖 胶原蛋白 

分 类 号：R135.1[医药卫生—劳动卫生]