肿瘤坏死因子α、X-连锁凋亡抑制蛋白在特发性胎儿生长受限中的表达  

Expressions of Tumor Necrosis Factor-α and X-Linked Inhibitor of Apoptosis Protein in Idiopathic Fetal Growth Restriction

作　　者：崔世红[1] 张婷[1] 韩笑[1] 程国梅[1] 杨健丽[1] 赵岚岚[1] 

机构地区：[1]郑州大学第三附属医院妇产科,郑州450052

出　　处：《实用儿科临床杂志》2012年第2期107-109,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省教育厅科技攻关项目(2010A320021)

摘　　要：目的探讨特发性胎儿生长受限(IFGR)患儿母血和脐血TNF-α及胎盘组织中X-连锁凋亡抑制蛋白(XIAP)的表达及其意义。方法选取郑州大学第三附属医院2010年7月-2011年7月行剖宫产分娩的IFGR孕妇34例作为实验组,同期因社会因素行剖宫产分娩的健康足月孕妇30例作为健康对照组。采用双抗体夹心酶联免疫吸附法(ELISA)测定2组母血、脐血TNF-α水平,免疫组织化学法检测2组胎盘组织中XIAP的表达。结果 1.实验组母血、脐血TNF-α水平[(90.12±6.43)μg.L-1,(98.73±7.29)μg.L-1]均明显高于健康对照组[(72.97±8.51)μg.L-1,(80.87±6.92)μg.L-1],差异均有统计学意义(Pa<0.05)。2.实验组XIAP在胎盘合体滋养细胞中的表达(114.56±5.17)明显低于健康对照组(144.40±7.31),差异有统计学意义(P<0.05)。3.实验组母血、脐血中TNF-α与胎盘组织中XIAP均呈负相关(Pa<0.05);健康对照组母血、脐血中TNF-α与胎盘组织中XIAP均无相关性(Pa>0.05)。4.实验组母血、脐血中TNF-α与新生儿体质量均呈负相关(Pa<0.05),健康对照组母血、脐血中TNF-α与新生儿体质量均无相关性(Pa>0.05)。5.实验组新生儿并发症显著高于健康对照组(P<0.05)。结论母血、脐血中TNF-α升高及胎盘组织中XIAP表达降低,可能通过促进胎盘滋养细胞过度凋亡参与IFGR的发病,并影响新生儿预后。Objective To investigate the expression and the significance of tumor necrosis factor-α（TNF-α） in maternal and cord serum,and X-linked inhibitor of apoptosis protein（XIAP）in placenta of idiopathic fetal growth restriction（IFGR）. Methods From Jul.2010 to Jul.2011,34 pregnant women having cesarean section with IFGR in the Third Affiliated Hospital of Zhengzhou University were selected as experimental group and 30 normal term pregnant women having cesarean section because of social factors were selected as control group.TNF-α in maternal and cord serum was analyzed by enzyme-linked immunosorbent assay.XIAP in placenta was detected by immunohistochemical SP method. Results 1.The levels of TNF-α in maternal and cord serum in the experimental group[（90.12±6.43） μg·L-1,（98.73±7.29） μg·L-1] were increased significantly higher than those in control group[（72.97±8.51） μg·L-1,（80.87±6.92） μg·L-1]（Pa0.05）.2.The expression of XIAP in placental trophoblast cells in experimental group was（114.56±5.17）,which decreased significantly,compared with control group（144.40±7.31）（P0.05）.3.The levels of TNF-α in maternal and cord serum were found in negative correlation with the expression of XIAP in placenta in experimental group（Pa0.05）,while the levels of TNF-α in the maternal and cord serum were found in no correlation with the expression of XIAP in placenta in control group（Pa0.05）.4.The levels of TNF-α in maternal and cord serum were found in negative correlation with the newborn birth weight in experimental group（Pa0.05）,while in control group,it was found in no correlation（Pa0.05）.5.Neonatal complications in experimental group were significantly higher than those in the control group（P0.05）. Conclusion The increased level of TNF-α in maternal and cord serum,decreased expression of XIAP in placenta may play an important role in the pathogenesis of IFGR through promoting excessive apoptosis of placental trophoblast cells,and that also affects prog

关 键 词：特发性胎儿生长受限 胎盘滋养细胞 肿瘤坏死因子-Α X-连锁凋亡抑制蛋白 

分 类 号：R346[医药卫生—基础医学]