头皮位点药物注射对宫内感染早产鼠脑髓鞘碱性蛋白、胶质纤维酸性蛋白及神经行为学的影响  

Effects of Scalp Sites of Drug Injection on Expressions of Myelin Basic Protein/Glial Fibrillary Acidic Protein and Neurobehavioral Change in Preterm Rats Caused by Intrauterine Infection

作　　者：李湘云[1] 栗艳芳[1] 高永强[1] 宋宗先[1] 王留霞[1] 

机构地区：[1]郑州大学第三附属医院脑瘫康复中心,郑州450052

出　　处：《实用儿科临床杂志》2012年第2期112-115,共4页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨头皮位点药物注射对宫内感染早产鼠脑组织髓鞘碱性蛋白(MBP)、胶质纤维酸性蛋白(GFAP)的表达及神经行为学的影响。方法 37只Wistar母鼠于孕16 d、17 d腹腔内注射脂多糖(LPS)或相同剂量的9 g.L-1盐水,将LPS组母鼠早产仔鼠(<孕22 d)于出生第7天分别予头皮位点注射VitB1、VitB12(A组)或丰富的环境干预(B组)或不做干预(C组);各组仔鼠于出生7 d、25 d采用免疫组织化学测脑组织MBP、GFAP蛋白的表达,并进行神经行为学检测。结果 7日龄LPS组早产仔鼠脑组织MBP蛋白表达(112.00±9.27)明显低于9 g.L-1盐水组(124.26±9.40)(P<0.01),大脑皮质及海马齿状回GFAP阳性细胞数目(39.45±4.70;22.55±3.91)明显高于9 g.L-1盐水组(34.36±3.72;18.82±3.25)(Pa<0.05);25日龄仔鼠中,脑组织MBP表达A组(138.79±9.21)高于C组(128.44±12.99)(P<0.01),B组(141.53±13.11)高于C组(P<0.01),GFAP阳性细胞数目大脑皮质B组(45.50±6.54)低于C组(51.42±6.99)(P<0.01),A组(46.70±5.61)低于C组(P<0.05),海马齿状回B组(35.35±3.70)低于C组(38.79±5.56)(P<0.05);悬吊试验得分,A组(3.65±0.22)分、B组(3.60±0.21)分高于C组(2.70±0.21)分(Pa<0.01);旷野试验中的得分,A组得分[(11.20±1.96)分]及B组得分[(10.80±1.96)分]均高于C组[(9.10±1.33)分](Pa<0.01),斜坡试验中B组试验时间[(2.19±0.74)s]最短,A组所用时间[(2.30±0.71)s]明显短于C组[(3.07±0.85)s](P<0.01)。结论宫内感染可诱发孕鼠早产并引起脑组织损伤;头皮位点药物注射可增加大鼠脑组织MBP的表达及抑制GFAP的过度表达,并改善宫内感染早产仔鼠的神经行为。Objective To investigate the effects of scalp sites of drug injection on expressions of myelin basic protein（MBP） / glial fibrillary acidic protein（GFAP） in brain tissue and neurobehavioral change in preterm rats caused by intrauterine infection. Methods Thirty-seven pregnant rats were subjected to intraperitoneal injection of lipopolysaccharide（LPS,350 μg·kg-1,n=32） or 9 g·L-1 saline（n=5） on gestation 16 d and 17 d.The preterm pups（birth before gestation 22 d） in the LPS group were respectively treated with scalp sites of VitB1,VitB12 injection（group A） or ample environment intervention（group B） or no intervention（group C）.Pups suffered from perfusion on 7 d and 25 d for measure of MBP and GFAP,and neurological behavior were processed before the sacrifice on 25 d. Results The expression of MBP in LPS 7 d pups（112.00±9.27） was significantly lower than that in 9 g·L-1 saline group（124.26±9.40）（P0.01）,whereas the GFAP positive cells both in cortex（39.45±4.70） and hippocampus dentate gyrus（DG）（22.55±3.91） were more than those in vehicle group（34.36±3.72;18.82±3.25）（Pa0.05）;In 25 d time point,group A（138.79±9.21） and group B（141.53±13.11） had much higher expression of MBP than that in group C（128.44±12.99）（Pa0.01）.The GFAP positive cells in cortex were 45.50±6.54 in group B,and 46.70±5.61 in group A,both of which were significantly less than that in group C（51.42±6.99）（P0.01,0.05）.Howe-ver,only group B had less GFAP positive cells in DG area（35.35±3.70） than that in group C（38.79±5.56）（P0.05）.In the neurolo-gic behavior,the scores of suspension tests in group A（3.65±0.22） were much hig-her than that in group C（2.70±0.21）（P0.01）,so was the group B（3.60±0.21）（P0.01）.Importantly,both group A（11.20±1.96） and group B（10.80±1.96） had a higher score in open field test than that in group C（9.10±1.33）,with a significant P value（Pa0.01）.In the slope test,group B offspri

关 键 词：位点药物注射 髓鞘碱性蛋白 胶质纤维酸性蛋白 神经行为 早产 

分 类 号：R722.6[医药卫生—儿科]