芹灵冲剂在2215细胞培养内对HBsAg,HBeAg的抑制作用  

Inhibition of Qinling Chongji on HBsAg and HBeAg secretion in cultured cell line 2215

作　　者：黄正明[1] 杨新波[1] 曹文斌[1] 陈鸿雁[1] 张敬珍[1] 滕丽[2] 陈鸿珊[2] 蔡光明[3] 刘东萍 

机构地区：[1]中国人民解放军北京军医学院药理教研室,北京市100071 [2]中国医学科学院医药生物技术研究所病毒室,北京市100050 [3]中国人民解放军302医院药理研究室,北京市100071

出　　处：《世界华人消化杂志》2000年第2期184-186,共3页World Chinese Journal of Digestology

基　　金：95军队重点科研基金面上A类课题;No.[1996]

摘　　要：目的 在乙型肝炎病毒（ＨＢＶ）基因的人肝癌细胞系（ＨｅｐＧ２的２２１５细胞培养中，观察芹灵冲剂（Ｑｉｎｌｉｎｇ Ｃｈｏｎｇｌｉ，ＱＬ）对细胞的毒性和对乙型肝炎病毒表面抗原（ＨＢｓＡＧ）和ｅ抗原（ＨＢｅＡｇ）的抑制效果。方法 （１）２２１５细胞培养。将配制（１×１０＾５个／ｍＬ）接种细胞培养板，９６孔板每孔０．１２ｍＬ，２４孔板每孔１ｍＬ，３７℃细胞毒笥试验：ＱＬ１２ｇ／Ｌ用２液２倍稀释成６个浓度，AIM To study the inhibiting effect of Qinling Chongji ( QL ) on HBsAg and HBeAg in the cultured hepatocarcinoma cell line 2215 (Hep G2) which was genetransfered with hepatitis·B virus (HBV) and its toxicity.METHODS Twenty-four hours after 2215 cells vaccination, the varied concentrations of QL were added to culture fluid, and exchanged the drugs every four days until to 8 d and 12 d. The cell alterations were observed under microscopy in order to evaluate the toxicity of QL. After treated with QL for 8 d and 12 d, the culture fluids were collected and the content of HBeAg and HBsAg was determined by using radioimmunoassay kits. RESULTS QL in nontoxic concentration significantly inhibited the secretion of HBsAg and HBeAg in cell line 2215 after 8 d and 12 d treatment. The mean inhibiting rate of QL (4000 μg·mL-1) on HBeAg was 51.57%±2.14% (8d) and 65.06%±1.94% (12 d), [ IC50 = (8 d) 3090.19 μg·mL-1±772.40 μg·mL-1 and (12 d) 907.13 μg·mL-1±93.57 μg·mL-1]; and was (8 d) 55.04% ±3.88% and (12d) 61.39%±0.98%, (IC50) = (8d) 2993.89 μg·mL-1±768.66 μg·mL-1 and (12 d) 1744.44 μg·mL-1±214.08 μg·mL-1 respectively. CONCLUSION The results show that QL can inhibit the secretion of HBsAg and HBeAg in cell line 2215 and has no toxicity at high concentration.

关 键 词：芹灵冲剂 肝肿瘤 HBSAG HBEAG 

分 类 号：R285[医药卫生—中药学]