利用昆虫病原线虫防治草菇双额岩小粪蝇  

Using Entomopathogenic Nematodes for Control of Mushroom Fly, Bifronsina bifrons (Stenhammar) (Diptera: Sphaeroceridae)

作　　者：刘桂清[1] 徐洁莲[1] 欧剑峰[1] 吴华[1] 黄鸿[1] 

机构地区：[1]广东省昆虫研究所,广州510260

出　　处：《中国生物防治学报》2012年第1期143-146,共4页Chinese Journal of Biological Control

基　　金：广东省科技成果转化启动项目(2010B060600008);广州市科技计划项目(2006Z3-E0711)

摘　　要：草菇双额岩小粪蝇Bifronsina bifrons是当前草菇生产中的重要害虫。本文测定了5个线虫品系在(30±0.5)℃下对草菇双额岩小粪蝇的致死率和LT50。结果表明,各线虫品系在浓度4000 IJs mL 1下的LT50范围为2.13～4.71 d,其中夜蛾斯氏线虫Steinernema feltiae SN品系对双额岩小粪蝇的毒力最大,LT50为2.13 d,侵染5 d后的致死率达86.67%。菇房施用4×106IJs.m 2S.feltiae SN品系和嗜菌异小杆线虫Heterorhabditisbacteriophora H06品系悬浮液于培养基表面6 d后,对双额岩小粪蝇的防治效果分别达到70.37%和45.68%,SN线虫对双额岩小粪蝇的防效与阿维菌素相当。Mushroom fly,Bifronsina bifrons,is an important insect pest in straw mushroom production in South China.Virulence of five strains of Steinernema and Heterorhabditis to the fly was bioassayed at（30±0.5） ℃ in laboratory.Results showed that LT50values of the five strains to the mushroom fly ranged from 2.13 d to 4.71 d.S.feltiae SN strain was a potential biocontrol agent against the mushroom fly with LT50value of 2.13 d and 86.67% of lethality at the concentration of 4000 IJs mL 1at 5 d after treatment.Field trails demonstrated that when nematode suspensions containing 4×106IJs m 2 were sprayed onto the mushroom beds the day before inoculation,control efficacy of S.feltiae SN strain and H.bacteriophora H06 strain against the mushroom fly at 6 d after treatment was 70.37% and 45.68%,respectively,and the control efficacy of S.feltiae SN strain was almost the same as that of avermectins.

关 键 词：昆虫病原线虫 双额岩小粪蝇 生物防治 草菇 

分 类 号：S436.462[农业科学—农业昆虫与害虫防治] S476.15[农业科学—植物保护]