多重耐药铜绿假单胞菌分子流行病学的研究  

The study of molecular epidemiology of multidrug-resistant pseudomonas aeruginosa clinical strains

作　　者：陈茶[1] 林冬玲[1] 李有强[1] 张妮[1] 袁慧[1] 曾建明[1] 张伟铮[1] 黄彬[2] 

机构地区：[1]广东省中医院检验医学部,广州510120 [2]中山大学附属第一医院检验医学部,广州510080

出　　处：《国际检验医学杂志》2011年第21期2427-2428,2432,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(81071397);广东省科技计划项目(2011B031800037);广东省医学科研基金资助项目(A2011168)

摘　　要：目的探讨广东省中医院72株多重耐药铜绿假单胞菌分子流行病学情况。方法采用纸片扩散法检测72株多重耐药铜绿假单胞菌对12种抗菌药物的敏感性,并用脉冲场凝胶电泳进行分子分型和同源性分析。结果 72株多重耐药铜绿假单胞菌均对3类或3类以上抗菌药物同时耐药,脉冲场凝胶电泳图谱可分成17基因型(A～Q),其中A型40株。结论本地区虽未发生暴发流行,但仍存在流行优势克隆株,应积极配合医院感染管理部门进行耐药性监测,防止医院感染和暴发流行。Objective To understand the molecular epidemiology of 72 multidrug-resistant pseudomonas aeruginosa isolates.Methods The sensitivity of 12 antibiotics were performed in 72 multidrug-resistant pseudomonas aeruginosa isolates by Kindy-Bauer method.Pulsed-field gel electrophoresis（PFGE） was performed to understand the molecular typing and homology analysis.Results 72 multidrug-resistant pseudomonas aeruginosa isolates were resistant to three or more than three types of antibiotics.Seventeen different PFGE types,designated from A to Q,were distinguished among these 72 isolates,40 clustered as the same type,designated type A.Conclusion The outbreak has not yet occurred in this region,but there are popular advantage clones.Therefore it required to monitor drug resistance actively cooperating with hospital infection control department,to prevent hospital infection and outbreak.

关 键 词：假单胞菌 铜绿 抗药性 多药 流行病学 分子 电泳 凝胶 脉冲场 

分 类 号：R181.3[医药卫生—流行病学]