电针足三里穴对失血性休克延迟补液大鼠肠组织缺血性损伤的影响  

Effects of electro-acupuncture of Zusanli points(足三里穴) on ischemia injury of gut in rats with delayed fluid replacement after hemorrhagic shock

作　　者：钟毓贤[1] 石现[2] 赵莹[1] 刘维维[1] 姚甲瑞[1] 张慧苹[1] 胡森[1] 

机构地区：[1]解放军总医院第一附属医院烧伤研究所休克与多器官功能障碍实验室,北京100048 [2]解放军总医院针灸科,北京100853

出　　处：《感染．炎症．修复》2011年第4期213-216,共4页Infection Inflammation Repair

基　　金：全军医学科研"十一五"专项课题(06Z055)

摘　　要：目的:研究电针足三里穴对失血性休克延迟补液大鼠肠组织缺血性损伤的影响。方法:30只SD雄性大鼠,按全血容量的40%放血制成失血性休克模型,随机分为3组:立即补液组(IFR)、电针非经非穴复合延迟补液组(SEA/DFR)和电针足三里穴复合延迟补液组(EA/DFR),每组10只。IFR组于失血后10 min即行补液,经股静脉输入2倍失血量的乳酸林格液。EA/DFR组于失血后10 min电针足三里穴(持续刺激25 min,4 Hz,4 V);SEA/DFR组电针非经非穴部位,其他操作同EA/DFR组:失血3 h以后两电针组均实施补液,补液方法同IFR组。测定失血前及失血后3和12 h的肠黏膜血流量(IMBF)、肠组织二胺氧化酶(DAO))活性及失血后12 h的空肠组织含水率。结果:失血后各组大鼠肠组织IMBF和DAO活性与失血前相比都显著降低(P均<0.05)。失血后3 h,EA/DFR组和IFR组的肠组织IMBF、DAO活性均显著高于SEA/DFR组,且EA/DFR组显著低于IFR组(P均<0.05)。失血后12 h,EA/DFR组和IFR组的肠组织IMBF、DAO水平均显著高于SEA/DFR组,肠组织含水率显著低于SEA/DFR组(P均<0.05);EA/DFR组的IMBF、肠组织含水率与IFR组比较差异无统计学意义(P均>0.05),DAO水平则显著低于IFR组(P<0.05)。结论:电针足三里穴对失血性休克延迟补液大鼠肠组织缺血性损伤有一定保护用。Objective： To investigate the effects of electro-acupuncture of Zusanli points （足三里穴） on ische- mia injury to gut in rats with delayed fluid replacement after hemorrhagic shock. Methods： Thirty SD male rats with 40% blood loss were randomly divided into 3 groups （n = 10）： immediate fluid resuscitation （IFR） group, delayed fluid resuscitation with sham electro-acupuncture group （SEA/DFR group）, and delayed fluid resuscita- tion with electro-acupuncture Zusanli points group （EA/DFR group）. IFR group received fluid replacement with Ringer＇s solution in volume 2 times of lost blood 10 minutes after blood loss. Electro-acupuncture was given to bi- lateral Zusanli points （ST36） with constant voltage （4 Hz, 4 V ） for 25 minutes approximately 10 minutes after blood loss,while no-acupuncture was conducted in SEA/DFR group. Two EA groups received delayed fluid resuscitation 3 hours after blood loss. The intestinal mucosal blood flow （IMBF） and diamine oxidase （DAO）were measured before hemorrhage, 3 hours and 24 hours after hemorrhage. The tissue water content of jejunum was also determined 12 hours after hemorrhage. Results.. The levels of IMBF and DAO in gut tissue were significantly decreased, the rate of tissue water content obviously increased after blood loss in all groups（all P〈0. 05）. Three hours after blood loss, IMBF and DAO levels of EA/DFR group and IFR group were significantly higher than those of SEA/DFR group, while IMBF and DAO of EA/DFR group were significantly lower than those of IFR group（all P〈0. 05）. Twelve hours after blood loss, IMBF and DAO levels were higher and the rate of tissue water content was lower in EA/DFR group and IFR group compared with those of the SEA/DFR group, IMBF and the rate of tissue water content of EA/DFR group showed no significant difference compared with those of IFR group （both P〈0.05）, while DAO levels of EA/DFR group were lower than that of IFR group（P〈0.05）. Conclusions：The results suggest that

关 键 词：休克 失血性 足三里穴 电针 肠 二胺氧化酶 局部血流量 

分 类 号：R589.2[医药卫生—内分泌]