不同产地树头菜中总黄酮含量的测定  

Determination of the Content of Total Flavonoids in Crateva unilocularis from Different Producing Areas

作　　者：何祯[1] 何明[2] 代向阳[2] 董琼[1] 

机构地区：[1]西南林业大学林学院西南山地森林资源保育与利用省部共建教育部重点实验室,云南昆明650224 [2]浙江农林大学林业与生物技术学院,浙江临安311300

出　　处：《安徽农业科学》2011年第22期13398-13399,共2页Journal of Anhui Agricultural Sciences

基　　金：西南林业大学重点基金项目(200906Z)

摘　　要：[目的]测定不同产地树头菜中总黄酮含量,为树头菜的优树筛选及推广提供技术支撑。[方法]以芦丁为对照品,采用亚硝酸钠-硝酸铝-氢氧化钠显色法,在510 nm处利用紫外分光光度法测定西双版纳,普洱,石屏,建水4个不同产地树头菜中总黄酮的含量。[结果]西双版纳,普洱,石屏,建水4个不同产地树头菜中总黄酮的含量分别为1.89、2.222、.02和1.49 mg/g,其中普洱树头菜中总黄酮含量最高,建水含量最低。[结论]该方法准确、可靠、重复性好,可以用作树头菜中总黄酮含量的测定。[Objective] To determine the content of total flavonoids in Crateva unilocularis from different producing areas.[Method] Using rutin as reference substance,contents of total flavonoids in Crateva unilocularis from different producing areas including Xishuangbanna,Puer,Shiping and Jianshui were determined at 510 nm by UV spectrophotometry colored with sodium nitrite-aluminum nitrate-sodium hydroxide.[Result] Contents of total flavonoids in Crateva unilocularis from Xishuangbanna,Puer,Shiping and Jianshui were respectively 1.89,2.22,2.02 and 1.49 mg/g,among which content of total flavonoids in Crateva unilocularis was the highest in Puer and lowest in Jianshui.[Conclusion] The method was accurate,reliable,repeatable and could be used to determine the content of total flavonoids in Crateva unilocularis.

关 键 词：树头菜(Crateva unilocularis) 总黄酮 紫外分光光度法 不同产地 

分 类 号：S567[农业科学—中草药栽培]