均匀设计法优选大黄酸提取工艺研究  

Study on the Extraction Process of Rhein from RADIX ET RHIZOMA RHEI by Uniform Design

作　　者：王杰[1] 李勇[1] 蒋斌[1] 魏群利[1] 

机构地区：[1]徐州医学院药学院,江苏徐州221006

出　　处：《安徽农业科学》2011年第22期13415-13416,共2页Journal of Anhui Agricultural Sciences

基　　金：徐州医学院院长专项人才基金(09KJZ23)

摘　　要：[目的]探讨提取相关因素与提取物中大黄酸含量的关系,研究大黄中大黄酸的最佳提取工艺。[方法]采用均匀设计法探讨以乙醇水为溶剂时,使用TS-NS系列小试型提取浓缩装置以粉碎度、乙醇浓度、固液比、提取时间为探讨因素,以大黄酸含量作为考察指标,来筛选大黄酸的最佳工艺研究。[结果]大黄酸优选工艺为85%乙醇作为提取溶剂,大黄颗粒过18目筛,固液比为1∶10,提取时间为0.5 h。[结论]该工艺科学、合理,可为以乙醇水为溶剂提取大黄中大黄酸的工艺优选提供科学依据。[Objective] To discuss the relationship between related factors and rhein content and optimize the extraction process of rhein from RADIX ET RHIZOMA RHEI by uniform design method.[Method] Taking the content of rhein as index,uniform design with 4 investigated factors including comminution degree,ethanol concentration,solid-liquid ratio,and extraction time was performed to optimize the extraction process of rhein from RADIX ET RHIZOMA RHEI with TS-NS extraction and concentration device.[Result] The extraction rate of rhein was the highest in extracts when RADIX ET RHIZOMA RHEI powder sieved with 18 meshes was extracted for 0.5 h with 85% ethanol as solvent under solid-liquid ratio of 1∶10.[Conclusion] The technology was scientific and reasonable that could provide a scientific basis for optimizing the extraction process of rhein from RADIX ET RHIZOMA RHEI using ethanol as extracting solvent.

关 键 词：大黄(RADIX ET RHIZOMA RHEI) 大黄酸 提取工艺 均匀设计法 

分 类 号：S567[农业科学—中草药栽培]