咪达唑仑与芬太尼联合应用对小鼠学习记忆的影响  

Effects of Combination of Midazolam and Fentanyl on the Learning and Memory in Mice

作　　者：晏明[1] 王玲[1] 韩孟和[1] 范洁[1] 李佳芹[1] 戴雷[1] 丁秋[1] 马涛[2] 刘金东[3] 

机构地区：[1]徐州医学院麻醉学院,江苏徐州221002 [2]徐州医学院麻醉药理学教研室,江苏徐州221004 [3]徐州医学院附属二院麻醉科,江苏徐州221002

出　　处：《中国临床医学》2011年第6期761-763,共3页Chinese Journal of Clinical Medicine

基　　金：2010年高等学校大学生实践创新训练计划项目(编号:2010798);浙江省宁波市医学科技计划项目(编号:2007014)

摘　　要：目的:探讨咪达唑仑与芬太尼合用对小鼠学习记忆的影响。方法:将80只小鼠分为避暗实验和跳台实验2大组,每个大组再分为4个亚组:对照组(C组)、咪达唑仑组(M组)、芬太尼组(F组)、咪达唑仑+芬太尼(M+F组),每组10只。应用避暗实验和跳台实验观察小鼠训练时潜伏期和错误次数,评估药物对小鼠学习记忆功能的影响。结果:24 h后,在避暗实验中,M+F组潜伏期较单用药组(M组或F组)缩短(P<0.05),但各组之间错误次数无显著差异(P>0.05);在跳台实验中,M+F组潜伏期显著短于单用药组(P<0.05),错误次数显著增多(P<0.05)。48 h后,在避暗实验和跳台实验中各组的潜伏期和错误次数均无显著差异(P>0.05)。结论:咪达唑仑与芬太尼合用能增强小鼠的遗忘作用,但用药24 h后作用消失,不会造成持久的学习记忆障碍。Objective：To investigate the impacts of combination of midazolam and fentanyl on learning and memory in mice. Methods：A total of 80 mice were divided into either step-through test group or step-down test group,each group was thereby divided into 4 subgroups（n = 10）：Control group（Group C）,midazolam group（Group M）,fentanyl group（Group F） and combination of midazolam and fentanyl group（Group M＋ F）.Latency and error times in each group were observed by step-through test and step-down test to evaluate the effects of medicines on learning and memory in mice.Results：Twenty-four hours after training,in step-through test,compared with Group F or Group M,the latency of Group M＋ F decreased（P〈0.05） but the error times did not change（P〈0.05）;in step-down test,the latency of Group M＋ F decreased（P〈0.05） and the error times increased（P〈0.05）.Forty-eight after training,there were no significant differences in the latency and error times in each group（P〉0.05）.Conclusions：Combination of midazolam and fentanyl can impair the learning and memory function during the medicines acting period,but the dysfunction disappears after 24h.It implies that the medicines cause no persistent dysfunction in learning and memory.

关 键 词：咪达唑仑 芬太尼 学习记忆 避暗实验 跳台实验 

分 类 号：R614.2[医药卫生—麻醉学]