正交试验优选红景天多糖的提取工艺  

Optimization of extraction technology of polysaccharides from Rhodiola rosea by orthogonal experiment

作　　者：黄冰洋[1] 魏海[1] 周权明[1] 郭靖[1] 钟丽婷[1] 姜艳霞[1] 

机构地区：[1]吉林医药学院基础医学院,吉林吉林132013

出　　处：《吉林医药学院学报》2011年第1期22-24,共3页Journal of Jilin Medical University

基　　金：吉林医药学院科研基金项目资助(2008003)

摘　　要：目的优选红景天多糖的最佳提取工艺。方法采用正交设计法筛选红景天多糖最佳提取工艺,用苯酚-硫酸比色法测定其含量。结果红景天多糖的最佳提取工艺为:提取的料液比1∶15,提取次数3次,提取时间1.5 h,乙醇浓度80%。测得红景天多糖的含量为7.57%,平均回收率98.84%,RSD为1.32%。结论该方法简便,准确,灵敏度高,适用于红景天多糖的提取和纯化。Objective To optimize the technology of polysaccharides extraction from Rhodiola rosea.Methods Orthogonal design was used to select optimum technology of Polysaccharides extraction from Rhodiola rosea and the content of total polysaccharides were detected by Phenol-vitriolic Colorimetry.Results Optimal extraction technology of Polysaccharide was as follow：solid-liquid ratio：1∶15;extraction time：1.5 hours;extraction times：3 times in total;ethanol concentration：80%.Polysaccharide content：7.57%,the average recovery rate：98.84%,and RSD：1.32%.Conclusion The method was simple,accurate,sensitive,and suitable for extraction and purification of polysaccharide from Rhodiola rosea.

关 键 词：红景天 多糖 正交设计法 

分 类 号：R284[医药卫生—中药学]