HPLC法测定广东土牛膝中二羟基泽兰素的含量  

Determination of Dihydroxyeuparin in Radix Eupatorii Chinensis by HPLC

作　　者：李瑞明[1] 张俊鹏[1] 任斌[1] 陈孝[1] 梁耀荣[1] 罗思婵[1] 喻丽元[1] 

机构地区：[1]中山大学附属第一医院药学部,广东广州510080

出　　处：《今日药学》2011年第10期617-619,共3页Pharmacy Today

基　　金：广东省医院药学研究基金(编号:2010A15);广东省自然科学基金面上项目(编号:8151008901000167)

摘　　要：目的建立广东土牛膝中二羟基泽兰素含量的高效液相测定方法。方法 采用高效液相色谱法,色谱柱为Diamonsil C18柱(250mm×4.6 mm,5μm),流动相为乙腈-0.2%(V/V)磷酸水梯度洗脱,流速为1.0 ml/min,检测波长240 nm。结果二羟基泽兰素在0.58～6.96μg/ml浓度范围内有良好的线性关系(r=0.999 8),平均加样回收率为99.65%,RSD为0.8%(n=6)。结论该方法简便、灵敏,准确,可用于广东土牛膝的质量控制。Objective To establish a method for the content determination of dihydroxyeuparin in Radix Eupatorii Chinensis by HPLC. Methods HPLC was developed to determine dihydroxyeuparin with UV absorptions at 240 nm. The analysis was performed on a Diamonsil C18 column （250 mm × 4. 6 mm,5 p.m） with a gradient solvent system of acetonitrile and aqueous phosphoric acid （0.2%, v/v） at a flow rate of 1.0 ml/min. Results There was a good linear relationship （r =0. 999 8） in the range of 0.58 -6.96μg/ml. The mean recovery was 99.65% ,RSD was 0.8% （n =6）. Conclusion This method is simple, sensitive and accurate, and can be used for quality control of Radix Eupatorii Chinensis.

关 键 词：广东土牛膝 二羟基泽兰素 HPLC 

分 类 号：R284[医药卫生—中药学]