CV-1细胞中固醇调节元件结合蛋白-1c对Ⅰ型葡萄糖载体mRNA稳定性及蛋白表达的影响  

Effects of sterol regulatory element binding protein-1c on stability of glucose transporter Ⅰ mRNA and it's protein expression in CV-1 cells

作　　者：李天洙[1] 常影[1] 柳明洙[1] 

机构地区：[1]延边大学基础医学院生物化学与分子生物学教研室,吉林延吉133002

出　　处：《延边大学医学学报》2011年第4期244-246,共3页Journal of Medical Science Yanbian University

基　　金：吉林省教育厅科学技术研究项目

摘　　要：[目的]探讨在CV-1细胞中固醇调节元件结合蛋白-1c(SREBP-1c)对Ⅰ型葡萄糖载体mRNA稳定性和蛋白表达的影响.[方法]利用DNA克隆技术,将SREBP-1c和其阴性表达体(ADDN)DNA序列重组到表达载体pSV-sport上.用SREBP-1c转染CV-1细胞,应用Northern Blot方法观察SREBP-1c对Ⅰ型葡萄糖载体mRNA的表达及其稳定性的影响,应用Western Blot方法观察SREBP-1c对Ⅰ型葡萄糖载体蛋白表达的影响.[结果]SREBP-1c可增加Ⅰ型葡萄糖载体的mRNA表达水平及稳定性,可提高Ⅰ型葡萄糖载体的蛋白表达水平.[结论]SREBP-1c可增强Ⅰ型葡萄糖载体的蛋白表达,可能通过调节Ⅰ型葡萄糖载体的表达水平而参与胰岛素对血糖的调节过程.OBJECTIVE To understand the effective of sterol regulatory element binding protein-1c（SREBP-1c） on stability of glucose transporter Ⅰ（GLUT1） mRNA and it＇s protein expression in CV-1 cells.METHODS SREBP-1c cDNA sequence and it＇s negative form（ADDN） were cloned into pSV-sport expression vector.The recombinant SREBP-1c and ADDN were transfected into CV-1 cells,and the stability of GLUT1 mRNA expression and protein expression were detected by time dependent and dose dependent with Northern Blot and Western Blot.RESULTS SREBP-1c stabilizeed the GLUT1 mRNA and increased the GLUT1 protein expression.CONCLUSION SREBP-1c increases GLUT1 expression,and it maybe participate in regulating process of blood glucose level through regulating GLUT1 expression controlled by insulin.

关 键 词：固醇调节元件结合蛋白质类 葡萄糖转运蛋白质类 易化性 RNA 信使 蛋白质类 

分 类 号：R34[医药卫生—基础医学]