中药水煎剂对粪肠球菌感染根管的体外抑菌试验  

The Experiment of Traditional Chinese Decoctioni' s Effect to Inhibit Enterococcus Faecalis Outside Body

作　　者：王贵花[1] 徐运强[1] 王荣霞[1] 

机构地区：[1]河北省石家庄市第二医院检验科,石家庄050051

出　　处：《中国中医药现代远程教育》2011年第21期137-138,共2页Chinese Medicine Modern Distance Education of China

基　　金：河北省卫生厅项目[No:20110184]

摘　　要：目的评价中药水煎剂对粪肠球菌的体外抑菌效果。方法选取70颗新鲜拔除的人单根管牙,截取6mm高的柱状牙根。将牙根随机分为阴性对照组、甲醛甲酚组(FC)、黄连水煎剂组、大黄水煎剂组、连翘水煎剂组、五味子水煎剂组、阳性对照组,每组10颗牙根。常规根管预备后灭菌制备感染粪肠球菌根管模型,阴性对照组不感染细菌,放置0.9%氯化钠注射液,阳性对照组感染细菌,放置0.9%氯化纳注射液,其余每组根管均感染细菌,分别放置上述不同药剂,培养3d,7d后分别测量细菌的透光度。结果大黄3d,黄连7d,FC7d无差别;五味子3d,阴性对照7d无差别;黄连3d,阴性对照3d无差别。结论相同浓度的中药水煎剂对粪肠球菌有不同程度的抑菌活性,黄连水煎剂的作用最强,其次是五味子,大黄,连翘作用最差。Objective The purpose to evaluate the effective of the traditional Chinese decoction＇ s function to inhibit enterococcus faecalis outside body.Method choose 70 fresh monoraldicular extracted off from patients,Intercept a fang of 6mm height.Divide randomly the fang into 7 groups which are fermiline control group,formaldehyde cresol formocresol group（FC）,coptis root decoction group,forsythia decoction group,coptis root decoction decoctiongroup,masculine control group,every group has 10 fangs.After preparing the common root canal and conduct germicidal treatment to it,and make infected enterococcus faecalis root canal models.Put 0.9% sodium chioride injection into fermiline non-infectious bacteria control group,put 0.9% sodium chioride injection into masculine infectious bacteria control group.Every other group of tool canals are infected bacterials.Put different medcine aboved,cultivate 3d,7d,then measure the bacterials＇ transmittancy.Result the rhubarb has no differences after 3day,coptis root after 7day,Fc after 7days,shizandra berry after 3days,fermiline control after 7 days;coptis root and fermiline control both have no differences after 3days.Conclution The traditional Chinese decoction of the same viscosity has different bacteriostatic activity to enterococcus faecalis.The effect of the coptis root decoction is the first,the coptis root decoction is the second,the rhubarb and forsythia are the worst.

关 键 词：中药水煎剂 粪肠球菌 体外抑菌 

分 类 号：R285.5[医药卫生—中药学]