柠檬黄对雄性小鼠生殖细胞的影响  

Toxic Effects of Tartrazine on Reproductive Cells in Male Mice

作　　者：蒋利刚[1] 程东[2] 韩晓英[1] 孟小倩[1] 景来东[1] 刘树真[1] 

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东省疾病预防控制中心,济南250014

出　　处：《生物医学工程研究》2011年第3期174-176,187,共4页Journal Of Biomedical Engineering Research

基　　金：山东省教育厅资助项目.00(J07YG02);山东省优秀中青年科学家科研奖励基金资助项目(2007BS02014;2008BS02016)

摘　　要：探讨柠檬黄对雄性小鼠生殖细胞的影响。选择成年雄性小鼠分别给予柠檬黄0.25 g/kg(低剂量组)、0.5 g/kg(中剂量组)和1 g/kg(高剂量组),连续灌胃染毒5 d,通过小鼠精子畸形率、精细胞微核率以及睾丸形态变化等方面来评价柠檬黄对生殖细胞的影响。与对照组相比,高剂量组的柠檬黄能引起小鼠精子的畸形率和精细胞微核率升高(P<0.05),而中、低剂量组没有明显的变化(P>0.05)。睾丸组织切片显示,低、中剂量组与对照组相比没有明显改变,高剂量组小鼠睾丸生精小管管腔内可见精子数量减少,生精上皮细胞层次减少,部分细胞有浓缩、溶解等坏死样变。高浓度的柠檬黄能使雄性小鼠精子畸形率增加,并造成雄性小鼠精细胞微核率上升,有一定的致突变性。To investigate the effects of tartrazine on reproductive cells in male mice.In order to research the toxicity effects of tartrazine on procreation cells of male mice,male adult mice were randomly divided into a control group,a tartrazine low-dosage group（0.25g/kg）,a tartrazine mid-dosage group（0.5g/kg） and a tartrazine high-dosage group（1g/kg）.All mice were exposed to tartrazine by gastic gavage for 5 days consecutively.The micronuclei rates of testicle cells,the spermatozoon monstrosity rates and testicle tissue were tested to explore the toxicity of reproductive cells.The results indicated that compared with the control group,micronucleus frequencies and spermatozoon monstrosity rates of male mice increased significantly in high-dosage groups（P0.05） and there was no significant difference among low-dosage,medium-dosage groups and the control group（P0.05）.There were no distinct histological changes in low-dosage and mid-dosage groups with comparison to those in the control group,however,in high-dosage group,lots of exutive spermatogenic cells were observed in the lumina of seminiferous tubule and the number of sperm cells were decreased.Since the spermatozoon monstrosity rates and micronucleus frequencies of male mice are raised by tartrazine,tartrazine can result in mutation to a certain extent.

关 键 词：柠檬黄 生殖细胞 微核 精子畸形 雄性小鼠 

分 类 号：R318[医药卫生—生物医学工程]