核桃青皮提取物对Hela细胞增殖及caspase-3蛋白表达的影响  

Effects of the green peel of carya cathayens on cell proliferation and caspase-3 expression in Hela cells

作　　者：陈默然[1] 赵行宇[2] 沈楠[3] 张巍[4] 

机构地区：[1]吉林医药学院招生就业处,吉林吉林132013 [2]吉林医药学院心理教研室,吉林吉林132013 [3]吉林医药学院实验中心,吉林吉林132013 [4]吉林医药学院生物化学教研室,吉林吉林132013

出　　处：《吉林医药学院学报》2011年第5期251-253,共3页Journal of Jilin Medical University

基　　金：吉林省教育厅"十一五"科学技术研究项目(吉教科合字2009-314;2011-286)

摘　　要：目的观察不同浓度核桃青皮提取物对Hela细胞增殖的抑制作用,探讨提取物对Hela细胞生长的影响。方法光镜下观察Hela细胞形态的改变,MTT法测定细胞生长抑制率,免疫组化观察caspase-3蛋白的表达。结果随提取物浓度的增加,细胞形态的改变明显,细胞变圆,折光率增加,细胞逐渐减少,对细胞生长的抑制作用增强,caspase-3蛋白的表达增加。结论提取物可抑制Hela细胞的生长,上调Hela细胞caspase-3蛋白的表达。Objective To study the effects of extract from the green peel of carya cathayensis on cell proliferation and easpase-3 expression in Hela ceils. Methods Hela cells were incubated with 25,50,100,200,400 mg/L of extract from the green peel of carya cathayensis for 24 h, respectively. Morphological changes was observed by light micro- scope, cell growth inhibition rate was determinated by MTT method and Casapse-3 expression was measured by Immu- nohistoehemistry. Results With the concentration of extract increased, the morphology of Hela cell had been changed obviously, the cell growth inhibition rate also increased, as well as caspase-3 protein expression.Conclusion Both cell proliferation and caspase-3 expression in Hela were affected by the extract from green peel of carya cathayen- sis were affected by the green peel of carya cathayens.

关 键 词：核桃皮提取物 HELA 细胞增殖 IC50 

分 类 号：R284.2[医药卫生—中药学]