补肾强脊颗粒对细胞因子诱导下的强直性脊柱炎成纤维细胞成骨型表达的影响  

Effect of Bushen Qiangqi Granule on Ossification Expression of Fibroblast of Ankylosing Spondylitis(AS) Induced by Cell Factor

作　　者：贾春蓉[1] 冯兴华[2] 刘宏潇[2] 李敏[2] 吴志奎[2] 

机构地区：[1]首都医科大学附属北京天坛医院,北京100050 [2]中国中医科学院广安门医院

出　　处：《浙江中医药大学学报》2011年第6期888-891,共4页Journal of Zhejiang Chinese Medical University

基　　金：国家自然科学基金资助课题(NO:30472277)~~

摘　　要：[目的]探讨补肾强脊颗粒对细胞因子诱导下的强直性脊柱炎(AS)成纤维细胞成骨型表达的影响。[方法]以体外培养的AS髋关节囊组织的成纤维细胞为实验对象,观察在细胞因子BMP-2与TGF-β的诱导刺激下,AS成纤维细胞成骨型表达标志物ALP活性、胶原含量、骨钙素含量的变化。[结果]细胞因子作用后,AS成纤维细胞的ALP活性、胶原含量、骨钙素含量较正常对照组与AS对照组有明显提高(P<0.01)。药物作用后,补肾强脊颗粒含药血清对细胞因子诱导下的AS成纤维细胞ALP、胶原含量、骨钙素含量活性有明显的抑制作用(P<0.01);而兔空白血清组SASP含药血清组则作用不明显(P>0.05)。[结论]补肾强脊颗粒可以通过抑制细胞因子对成纤维细胞成骨型标志物的影响而达到抑制成纤维细胞进一步向成骨型的分化。[Objective] To explore the effect of Bushen Qiangqi Granule on ossification expression of fibroblast of AS induced by cell factor.[Method] Take fibroblast of AS hip joint bag tissue cultured in vitro as experimental object,observe the change of AS fibroblast ossification expression landmark ALP activity,collagen content and BGP.[Result] Functioned by cell factor,the changes are much raised compared with control group.Functioned with medicine,Bushen Qiangqi Granule medicated serum has inhibition to fibroblast ALP,collagen content and BGP activity;while in rabbit blank serum group,SASP medicated serum is functioned little.[Conclusion] Bushen Qiangqi Granule can inhibit fibroblast from further ossification by inhibiting cell factor influencing on ossification landmark.

关 键 词：强直性脊柱炎 补肾强脊颗粒 细胞因子 骨化 成纤维细胞 

分 类 号：R651.2[医药卫生—外科学]