RP-HPLC法测定总东莨菪内酯血药浓度  

Determination of Plasma Concentration of Total Scopoletin by RP-HPLC

作　　者：魏敏[1] 周莉玲[1] 李锐[1] 

机构地区：[1]广州中医药大学,广州510405

出　　处：《中药新药与临床药理》2000年第1期11-13,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省自然科学基金!(980 63 9)

摘　　要：用PR-HPLC法测定丁公藤注射液有效成分总东莨菪内酯在小鼠血浆中的浓度。色谱条件:SpheriSorb_C18不锈钢柱,流动相为甲醇-水-冰醋酸(38∶62∶0.19),检测波长345nm。血浆样品经酸水解后用氯仿萃取,50℃水浴,氮气吹干,甲醇超声溶解后进样。本法具有快速、简便、灵敏、准确等特点,已经用于总东莨菪内酯血药浓度测定及药代动力学研究,结果满意。The plasma concentration of total scopoletin in Dinggongteng Injection was determined by RP_HPLC. The chromatographic conditions were as follows: stainless column Spherisorb C 18 ; mobile phase:methanol_water_glacial acetic acid (38∶12∶ 0.19 ); detection wave length 345nm. The plasma sample was extracted by chloroform after acid hydrolysis, dried by nitrogen on a water bath at 50℃, and dissolved with methanol. This method is rapid simple, sensitive and accurate, and has been used in plasma concentration determination and pharmacokinetics of total scopoletin.

关 键 词：丁公藤注射液 东莨菪内酯 药代动力学 血浆 

分 类 号：R969.1[医药卫生—药理学]