水芹乙酸乙酯提取物在2215细胞培养内对乙型肝炎病毒表面抗原和E抗原的抑制作用  

Inhibition of Sthyl Autate extract of oenanthe javanica on secreti of HBsAg and HBeAg in cultured cell line 2215(Hep G 2)

作　　者：杨新波[1] 黄正明[1] 曹文斌[1] 陈鸿雁[1] 张敬珍[1] 陈鸿珊[2] 滕丽[2] 刘东萍 

机构地区：[1]中国人民解放军北京军医学院药理教研室,北京100071 [2]中国医学科学院医药生物技术研究所病毒室,北京100050 [3]中国人民解放军302医院药理研究室,北京100071

出　　处：《解放军药学学报》2000年第1期4-8,共5页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家新药基金!资助项目;课题编号96 - 901 - 05 - 16A

摘　　要：目的：观察水芹乙酸乙酯提取物（简称水芹提取物）在乙型肝炎病毒（ＨＢＶ）基因转染的人肝癌细胞系（ＨｅｐＧ２）２２１５细胞培养中，对细胞的毒性及对ＨＢＶ表面抗原（ＨＢｓＡｇ）和ｅ抗原（ＨＢｅＡｇ）的抑制效果。方法：水芹提取物用培养液稀释成２０００、１０００、５００、２５０、１２５μｇ·ｍｌ－１浓度，分别加入２２１５细胞内培养１２ｄ，观察药物对细胞的毒性。在无毒浓度下的水芹提取物，以培养液稀释１０００、５００、２５０μｇ·ｍｌ－１３浓度，分别加入２２１５细胞内培养，于第６、９ｄ收集的培养液，用固相放射免疫法测定ＨＢｓＡｇ和ＨＢｅＡｇ，γ－计数器测定每孔药液ｃｐｍ值。结果：对细胞的半数中毒浓度（ＴＣ５０）平均为２２８４．７３±１２７．３５μｇ·ｍｌ－１，最大无毒浓度为１０００μｇ·ｍｌ－１。大、中剂量（１０００、５００μｇ·ｍｌ－１）对２２１５细胞分泌的ＨＢｓＡｇ、ＨＢｅＡｇ的活性于试验的第６、９ｄ均有明显的抑制作用（Ｐ＜０．０５，Ｐ＜０．０１，Ｐ＜０．００１），并有一定转阴作用。结论：水芹提取物在２２１５细胞中培养６～９ｄ，最大无毒浓度可明显抑制ＨＢｅＡｇ和ＨＢｓＡｇ的分泌。Aim To sduty the inhibitory effects of Ethyl Acetate Extract of Oenanthe Javanica on HbsAg and HbeAg and its toxicity in the cultured hepatocarcinomaline 2215 (Hep G 2) which was genetrasfered with hepatitis B virus.Methods Twenty-four hours after 2215 cells vaccinated,the different concentrations of Ethyl Acetate Extract of Oenanthe Javanica (2 000,1 000,500,250,125μg·ml -1 ) were added into culture fluid respectely, and exchanging the drugs every four days within 12 days.The cell alteration was observed under microcopy in order to evaluate the toxicity of Ethyl Acetate Extract of Oenanthe Javanica.The cells were treated with Ethyl Acetate Extract of Oenanthe Javanica (no toxicity concentration:1 000,500,250μg·ml -1 ) for 6 days and 9 days, culture fluid was collected and the contents of HBeAg and HBsAg were determined using radioimmunoassay kits respectively.Results Three seperated expirements showed that the mean half toxicity concentration of Ethyl Acetate Extract of Oenanthe Javanica was 2 284.73+127.35μg·ml -1 ) and its maximum of no toxicity concentration was 1 000μg·ml -1 ).Ethyl Acetate Extract of Oenanthe Javanica (maximum of no toxicity concentration:1 000,500μg·ml -1 ) significantly inhibited the secretion of HBeAg and HbsAg in cell line 2215 after 6 days and 9 days treatment(P<0.05 and P<0.01,P<0.001).Conclusion The results show that the maximum of no toxicity concentration of Ethyl Acetate Extract of Oenanthe Javanica can inhibite the secretion of HBeAg and HbsAg in cell line 2215 after 6 and 9 days.

关 键 词：水芹 乙酸乙酯提取物 表面抗原 HBEAG HBsAg 

分 类 号：R978.7[医药卫生—药品] R965.1[医药卫生—药学]