4种滑叶铁线莲基因组DNA提取方法比较  

Comparison on Four Extraction Methods of Genomic DNA from Clematis fasciculiflora Franch

作　　者：胡祎晨[1] 孙正海[1] 王锦[1] 李世峰[2] 辛培尧[1] 范萱[1] 

机构地区：[1]西南林业大学园林学院,云南昆明650224 [2]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《安徽农业科学》2011年第36期22215-22216,22363,共3页Journal of Anhui Agricultural Sciences

基　　金：云南省应用基础研究项目(2010ZC089);国家林业局948项目(2008-4-11);云南省省部级重点学科;省高校重点实验室及校实验室共享平台资助项目;西南林业大学科技创新基金

摘　　要：[目的]对4种滑叶铁线莲基因组DNA提取方法进行比较研究,建立滑叶铁线莲最适DNA提取方法。[方法]以滑叶铁线莲叶片为材料,比较改良CTAB法Ⅰ、改良CTAB法Ⅱ、改良CTAB法Ⅲ、改良SDS法这4种基因组DNA提取法在提取DNA纯度、浓度和提取时间等方面的不同。[结果]4种方法都可提取滑叶铁线莲基因组DNA。改良CTAB法Ⅰ提取DNA纯度最高,但浓度最低且提取时间最长;改良SDS法提取DNA浓度最高,所需时间较短,但纯度较低;改良CTAB法Ⅲ提取所需时间最短。[结论]建立了铁线莲最适DNA提取方法,为运用分子生物学手段对其研究提供支持。[ Objective ] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematisfasciculiflora Franch. [ Method] Leavies of Clematisfas- ciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method I , improved CTAB method ]I, improved CTAB method 111 and improved SDS method. [ Resultl The four extraction methods could all successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch. The purity of genomic DNA was the highest using improved CTAB method I , with the longest extracting time ; while the concentration of genomic DNA was the maximum using the improved SDS method, with the shortest extracting time and relatively low purity; the extracting time of improved CTAB method m was the shortest. [ Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.

关 键 词：滑叶铁线莲 基因组DNA提取 改良CTAB法 改良SDS法 

分 类 号：S188[农业科学—农业基础科学]