Smad7和组蛋白去乙酰化酶1在小鼠马兜铃酸肾病中的表达  

Expression of Smad7 and histone deacetylase 1 in the mouse with aristolochic acid nephropathy

作　　者：彭晓兰[1] 董晓凯[1] 廖芳芳[1] 高磊[1] 张中文[1] 吴国娟[1] 

机构地区：[1]北京农学院动物科技学院,北京102206

出　　处：《解剖学报》2012年第1期88-92,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(31172362;30972215);北京市自然科学基金资助项目(5102014;6092004);北京市属高校人才强教计划资助项目(PHR2009-07135);北京市教委资助项目(KM 201010020008)

摘　　要：目的探讨Smad7、组蛋白去乙酰化酶1(HDAC1)在小鼠马兜铃酸肾病(AAN)中的表达及两者在马兜铃酸肾病发生、发展中的作用。方法将32只体重(20±2)g的SPF(无菌)雄性昆明小鼠随机分为4组,其中3组为实验组,灌胃给予不同剂量的马兜铃酸(AA)Ⅰ,分别为4 mg/(kg.d)、8 mg/(kg.d)、16mg/(kg.d)。另设对照组,PBS灌胃。应用免疫组织化学、免疫印迹法及RT-PCR技术,检测AAN小鼠和正常对照组中Smad7和HDAC1的表达情况。结果 HDAC1阳性着色主要分布于细胞核上,且在AAN中的表达强度明显高于正常对照组。免疫印迹法检测和RT-PCR提示,Smad7在AAN中的表达低于对照组,而HDAC1的表达显著高于对照组。结论在AAN中Smad7低表达以及HDAC1高表达,提示Smad7和HDAC1可能在AAN的发生、发展中起重要作用。Objective To investigate the roles of Smad7 and histone deacetylase 1 （ tIDACI ） in the devclopment of aristolochic acid nephropathy （AAN） , we studied their expressions in AAN. Methods The expressions of Smad7 and HDACI in the AAN and control groups were measured by immunohistochemical technique, Western blotting and RT- PCR. A total of 32 male mice were randomized into four groups. Aristolochic acid（AA） I was dissolved in PBS and was given to mice in three treatment groups through intragastric administration [4 mg/（kg-d） , 8 rag/（kg-d） , and 16 rag/ （kg. d） , respectively]. Results The immunohistochemical positive reaction for HDAC1 was distributed in the epithelial cell nuclei of the renal tubules. The expression level of HDAC1 was significantly higher in the AAN group than that in the control group. The results of Western blotting and RT-PCR showed that the protein expression of Smad7 in the AAN group was significantly lower than that of the control group, while the situation was opposite for HDACI expression. Conclusion The higher expression of HDAC1 and lower expression of Smad7 in AAN indicated that HDAC1 and Smad7 may play an important role in the development of AAN.

关 键 词：马兜铃酸肾病 SMAD7 组蛋白去乙酰化酶1 免疫组织化学 免疫印迹法 小鼠 

分 类 号：R965.1[医药卫生—药理学]