原发性干燥综合征患者外周血单个核细胞内肿瘤坏死因子受体相关因子6的表达及意义  

TNF receptor-associated factor 6 expression in peripheral blood mononuclear cells of patients with primary Sjgren's syndrome and its clinical significance

作　　者：秦琴[1] 张建荣[1] 谷明莉[1] 胡志德[2] 赵东宝[3] 邓安梅[1] 

机构地区：[1]第二军医大学长海医院实验诊断科,上海200433 [2]济南军区总医院实验诊断科,济南250031 [3]第二军医大学长海医院风湿免疫科,上海200433

出　　处：《第二军医大学学报》2012年第1期63-66,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30972730);上海市科委基金(09JC1405400)~~

摘　　要：目的研究肿瘤坏死因子受体相关因子6(TRAF6)在原发性干燥综合征(pSS)患者外周血单个核细胞(PB-MCs)中的表达及意义。方法采用实时荧光定量反转录PCR法检测了23例pSS患者和23例健康对照个体PBMCs中TRAF6mRNA的相对表达量,分析了其与pSS患者血清类风湿因子(RF)、免疫球蛋白浓度、抗SSA抗体和抗SSB抗体的关系。对其中9名接受糖皮质激素治疗的pSS患者进行了随访,观察了治疗前后患者PBMCs中TRAF6mRNA水平的变化。结果 pSS患者PBMCs中TRAF6mRNA的相对表达量是健康对照个体的2.77倍(P<0.01),且与血清RF(r=0.45,P=0.03)和免疫球蛋白水平(r=0.43,P=0.04)呈正相关,但与抗SSA抗体和抗SSB抗体无关。9名患者经过治疗后TRAF6mRNA表达下调了约50%(P<0.05)。结论 PBMCs中的TRAF6参与了pSS的发病机制,且是一种潜在的pSS标志物。Objective To investigate the expression of TNF receptor-associated factor 6 （TRAF6） in the peripheral blood mononuclear cells （PBMCs） of primary Sjogren＇ s syndrome （pSS） patients and its clinical significance. Methods The expression of TRAF6 mRNA was determined by quantified reverse transcriptional PCR in the PBMCs of 23 pSS patients and 23 healthy controls. The correlation of TRAF6 mRNA level with the laboratory findings, including serum rheumatoid factor （RF）, globulin, anti-SSA and anti-SSA antibody, were analyzed by Spearman test. Nine of the pSS patients were followed up and their TRAF6 mRNA expression was detected after glucocorticoid treatment for 1-2 months. Results The TRAF6 mRNA level in pSS patients was 2. 77 times higher than that in the healthy controls（P（0. 01）. The TRAF6 mRNA level in pSS patients was positively correlated with serum RF （r = 0. 45, P = 0. 03） and globulin （r = 0. 43, P = 0. 04）, but was not associated the anti-SSB and anti-SSA antibody. TRAF6 mRNA level was decreased by about 50% in the patients who were treated with glucocorticoids for 1-2 months （P（0.05）. Conclusion TRAF6 in PBMCs is involved in the pathogenesis of pSS and it may be a potential biomarker for pSS.

关 键 词：干燥综合征 肿瘤坏死因子受体相关因子6 单核白细胞 

分 类 号：R593.2[医药卫生—内科学]