口服螺内酯对SHR心肌组织TNF-α表达的影响  

Effects of Oral Spironolactone on TNF-α Expression of Myocardial Tissues in Spontaneous Hypertension Rats

作　　者：赵红[1] 李海涛[1] 顾定伟[1] 李素新[1] 方艳淑[1] 韩春萍[1] 

机构地区：[1]河北联合大学附属医院心血管科,唐山063000

出　　处：《天津医药》2012年第1期64-66,共3页Tianjin Medical Journal

基　　金：河北省教育厅自然科学基金资助项目(项目编号:2004119)

摘　　要：目的:观察口服小剂量螺内酯对自发性高血压大鼠(SHR)心肌组织TNF-α表达水平的影响。方法:20只8周龄雄性SHR随机分为螺内酯组和安慰剂组,每组10只,另设对照组(Wistar-kyoto,n=7)。螺内酯组螺内酯双蒸水溶解,20mg/(kg.d)灌胃,安慰剂组和对照组等容积双蒸水灌胃,持续16周,24周龄大鼠称取体质量,颈动脉插管检测动脉血压。摘取心脏,心尖部横断为两片,分别投入液氮罐中和10%中性甲醛固定液中,用ELASA方法检测心肌组织匀浆上清液TNF-α水平,免疫组化观察TNF-α表达。结果:螺内酯组与安慰剂组比较,收缩压、舒张压、平均动脉压水平无明显变化(P>0.05),但两组的收缩压、舒张压、平均动脉压均明显高于对照组,差异有统计学意义(P<0.05)。安慰剂组与对照组比较,心肌组织TNF-α水平明显升高,螺内酯组与安慰剂组比较心肌组织TNF-α水平降低,差异有统计学意义(P<0.01),但未达对照组水平(P>0.05)。结论:SHR大鼠心肌组织TNF-α高表达,口服小剂量螺内酯可降低SHR大鼠心肌组织TNF-α水平。Objective：To investigate the effect of low-dose oral spironolactone（SPIRO） on the expression of TNF-α of myocardial tissues in spontaneous hypertension rats（SHR）.Methods：Twenty 8-week-old male SHR were randomly divided into SPIRO group（SHR-SPIRO,n=10） and control（CON） group（SHR-CON,n=10）.Seven sex-age matched Wistar Kyoto rats（WKY group） were used as the third group.The SPIRO was dissolved in distilled water,and animals were intragastrically administrated SPIRO and weighed once weekly to adjust the drug dosage [20 mg/（kg·d）].The evaluation was performed after 16 weeks of treatment.The rats were anesthetized with 1 g/kg urethane intraperitoneally at 24-week-old.The systolic pressure,diastolic pressure and mean arterial blood pressure were detected by a tube that was pierced into the carotid artery and was connected with physiological function experimental system.The hearts of rats were rapidly isolated and were cut into two parts.One was fixed in 10% buffered formalin fixation solution for immunohistochemistry examination,the other one was thrown quickly into liquid nitrogen.The TNF-α level of left ventricular homogenate was detected by enzyme linked immunosorbent assay.Results：There were no significant differences in values of systolic blood pressure,diastolic blood pressure and mean arterial pressure between SHR-SPIRO group and SHR-CON group（P 0.05）.But there were significantly higher systolic blood pressure,diastolic blood pressure and mean arterial pressure in the two groups compared to those of WKY group（P 0.05）.The concentration of TNF-α in myocardial tissues was significantly higher in SHR-CON group than that of WKY group（P 0.01）.The level of TNF-α expression in myocardial tissues decreased significantly in SHR-SPIRO group than that of SHR-CON group（P 0.01）.Conclusion：The level of TNF-α expression in myocardial tissues increased in hypertension rats.The low-dose oral spironolactone can decrease the concentration of TNF-α of myocardial tissues in hyp

关 键 词：醛固酮 螺内酯 肿瘤坏死因子α 心内膜心肌纤维化症 大鼠 近交SHR 

分 类 号：R285.5[医药卫生—中药学]