骨髓Sca-1^+间充质干细胞移植减轻小鼠迟发型超敏反应  

Sca-1^+ bone marrow mesenchymal stem cells transplantation for the alleviation of delayed type hypersensitivity in mouse

作　　者：曲学彬[1] 曾洋[1] 刘星霞[1] 赵春华[1] 

机构地区：[1]中国医学科学院北京协和医学院基础医学研究所组织工程中心,北京100005

出　　处：《基础医学与临床》2012年第2期133-137,共5页Basic and Clinical Medicine

摘　　要：目的探讨骨髓Sca-1+间充质干细胞(BMSCs)在迟发型超敏反应(DTH)小鼠中的免疫调控作用。方法 BALB/c小鼠随机分为对照组及MSCs注射组。注射组于0、2及6 d腹腔注射Sca-1+BMSCs,对照组注射0.9%氯化钠注射液。所有小鼠在7和14 d时分别于背部皮下与足跖注射C57BL/6小鼠脾细胞。24 h后用千分尺测量小鼠足跖的肿胀,ELISA法测外周血细胞因子及FACS法测脾脏免疫细胞比例。结果注射组小鼠足跖的肿胀程度[(0.368±0.126)mm]显著轻于对照组[(0.731±0.111)mm,P<0.01]。注射组外周血中IL-10和TGF-β含量显著高于对照组(P<0.05);而IL-12和TNF-α水平显著低于对照组(P<0.05)。注射组小鼠脾脏调节型T细胞和树突细胞的比例均明显高于对照组(P<0.05)。结论 BMSC通过上调抑炎因子和调节型免疫细胞的数量而减轻DTH小鼠的免疫反应。Objective To observe the immunoregulatory effects of Sca-1 ＊ bone marrow mesenchymal stem cells （BMSCs） on the delayed type hypersensitivity （DTH） mouse. Methods BALB/c mice were randomly divided in- to two groups, control group and MSCs infusion group. MSCs infusion group mice were intraperitoneally injected with Sca-1 ~ BMSCs from Balb/c mice on O, 2 and 6 d, while control group mice were injected with normal saline. All the recipient mice were then injected with C57BL/6 splenocytes into the dorsal flank on 7 and 14 d into the hind footpad. After 24 h, footpad thickness, cytokines levels in the peripheral blood and the proportion of regulato- ry immune cells in spleen were measured by micrometer, ELISA and FACS, respectively. Results Footpad thick- ness of MSCs infusion group mice [ （0. 368 ±0. 126）mm] was significantly lower than that in the control group [ （0. 73l ±0. 111）mm, P 〈0. 01 ]. In the peripheral blood of MSCs infusion group mice, levels of IL-10 and TGF-J3 were higher than that of control group mice; while levels of IL-12 and TNF-et were lower than that of control group （P 〈 0. 05 ）. The proportion of ded that of control group mice （ P 〈 0. regulatory T cells and dendritic cells in the MSCs infusion group mice excee- 05）. Conclusions Transplantation of BMSCs alleviated DTH in vivo via upregulating the levels of anti-inflammatory cytokines and regulatory immunocytes.

关 键 词：间充质干细胞 迟发型超敏反应 免疫反应 

分 类 号：R392.4[医药卫生—免疫学]