Slug减轻照射鼠肠上皮细胞损伤及作用机制  

The protection effect and mechanism of Slug on irradiation of rat intestinal epithelial cells

作　　者：迟绍云[1] 焦学龙[2] 王宝泉[1] 刘春生[1] 王蕾[1] 陈栋[2] 

机构地区：[1]青岛大学医学院附属海阳市人民医院普外科,山东海阳265100 [2]青岛大学医学院附属医院普外科,山东青岛266003

出　　处：《基础医学与临床》2012年第2期138-142,共5页Basic and Clinical Medicine

摘　　要：目的研究Slug过表达对照射鼠肠上皮细胞的保护作用及机制。方法以体外培养的小肠隐窝上皮细胞IEC6和转染了Slug cDNA的IEC6为研究对象,对细胞进行6 Gy剂量γ射线照射,在照射48 h后观察细胞凋亡指数,免疫组化和Weatern blot法检测PUMA蛋白表达。结果 6 Gy射线照射IEC6细胞48 h后的细胞凋亡指数为15.60±1.54,明显高于照射联合pcDNA3-Slug cDNA转染组的5.10±1.70(P<0.01)和单独pcDNA3-Slug cDNA转染组的2.21±0.36(P<0.01)。6 Gy射线照射IEC6细胞48 h后的PUMA相对表达为0.79±0.12,明显高于照射联合pcDNA3-Slug cDNA转染组的0.16±0.01(P<0.01),免疫组化和Weatern blot法检测结果一致。结论上调Slug抑制射线诱导的细胞PUMA表达上调,抑制IEC6细胞凋亡。Objective To investigate the protection effect and mechanism of slug overexpression on irradiation of rat intestinal epithelial cells. Methods The intestinal crypt epithelial cells （IEC6） were used and transfected with Slug cDNA in vitro. The cells were irradiated with 6 Gy 60 Co gamma rays. 48 hours after the irradiation, apoptosis indexes were calculated. The expression of PUMA was observed by the immunohistochemistry and Western blot.Results The apoptosis indexes in IEC6 by the irradiation of 60Co gamma rays after 48 hours were significantly high- er than that in peDNA3-Slug eDNA and in pcDNA3-Slug eDNA without irradiation（ 15.60 ± 1.54 versus 5.10± 1.70; 2. 21±0.36, P 〈0. 01 ）. The expression of PUMA in IEC6 by the irradiation of 60Co gamma rays after 48 hours was significantly higher than that in pcDNA3-Slug eDNA by Western-blot （0. 79 ＋ 0. 12 versus 0. 16 ± 0. 01, P 〈0. 01 ）. The immunohistochemistry and Western blot assay results were consistent. Conclusions Upregulation of Slug can restrain the upregulation expression of PUMA in irradiated cells and the apoptosis of IEC6.

关 键 词：SLUG PUMA 射线照射 凋亡 鼠肠上皮细胞 

分 类 号：R818[医药卫生—放射医学]