不同光质对烟草叶片生长发育过程中类半胱氨酸蛋白酶活性及其基因表达调控的影响  

Effect of Different Light Qualities on the Activities of Caspase-like Proteases and the Regulation of Gene Expression during Growth and Development of Tobacco Leaves

作　　者：赵娟[1,2,3] 柯学[1,2,3,4] 徐超华[1,2,3] 李军营[5] 龚明[1,2,3] 

机构地区：[1]云南师范大学生命科学学院,云南昆明650092 [2]生物能源持续开发利用教育部工程研究中心,云南昆明650092 [3]云南省生物质能与环境生物技术重点实验室,云南昆明650092 [4]中国农业大学水利与土木工程学院,北京100083 [5]云南省烟草农业科学研究院,云南玉溪653100

出　　处：《安徽农业科学》2011年第23期13965-13968,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30460016);中国烟草总公司云南省公司科技计划项目(2010YN03;07A01)

摘　　要：[目的]探讨不同光质对烟草叶片生长发育及衰老进程中类半胱氨酸蛋白酶活性及其基因表达的调控。[方法]通过覆盖不同颜色滤膜(白膜、红膜、黄膜、蓝膜和紫膜)获得不同光质,监测不同光质下烟草叶片在生长发育及衰老过程中叶绿素含量、类半胱氨酸蛋白酶活性及相关几个基因表达的变化。[结果]与白、红、黄膜处理相比,蓝膜和紫膜处理延缓了烟草叶片生长后期叶绿素含量的下降和衰老进程,同时蓝、紫膜处理的烟草叶片有相对较低的类半胱氨酸蛋白酶活性和相关的几个基因的表达量。[结论]不同光质对烟草叶片的生长发育及衰老进程有不同的影响,这在某种程度上是通过影响类半胱氨酸蛋白酶及其相应的基因表达来实现的。[Objective] The study aimed to investigate effects of different light qualities on the regulation of activity and gene expression of caspase-like proteases during growth,development and senescence of tobacco leaves.[Method] By covering tobacco plants with white,red,yellow,blue and purple films to obtain different light qualities,the changes of chlorophyll content,activity and gene expression of caspase-like proteases were studied in the tobacco leaves.[Results] As comparing with white,red and yellow film treatments,blue and purple film treatments delayed the decrease of chlorophyll content and senescence of tobacco leaves at the late growth stage,and relatively lowered the activity and related gene expression of caspase-like proteases during growth,development and senescence of tobacco leaves.[Conclusion] The different light qualities applied various effects on growth,development and senescence of tobacco leaves,possibly by affecting the activity and gene expression of caspase-like proteases to some extent.

关 键 词：光质 烟草叶片 生长发育 类半胱氨酸蛋白酶 基因表达 

分 类 号：S572[农业科学—烟草工业]