氢化可的松对小鼠T淋巴细胞增殖的抑制作用  

The Inhibitory effects of Hydrocortisone on Proliferation of Mouse T Lymphocytes

作　　者：彭新青[1] 周大杰 乔玉莉[1] 韦日明[1] 

机构地区：[1]桂林医学院生物技术学院,广西桂林541004

出　　处：《安徽农业科学》2011年第23期14112-14112,14161,共2页Journal of Anhui Agricultural Sciences

基　　金：广西教育厅科研立项项目(桂教科研34号;200710LX354)

摘　　要：[目的]探讨氢化可的松对小鼠T淋巴细胞增殖功能的影响。[方法]用淋巴细胞分离液无菌制备小鼠脾淋巴细胞,于RPMI1640的完全培养基中加入ConA后进行转化。用流式细胞仪检测T细胞活化分子CD25和CD69及其共表达率。将转化的淋巴细胞分成不加氢化可的松的对照组和加入不同浓度的氢化可的松组,在各时间点取样计数,绘制其生长曲线。[结果]经ConA刺激3 d的T细胞上CD25和CD69分子及其共表达率最高,分别达73.5%、58%和32%。生长曲线显示,各组细胞在加氢化可的松后第2 d达到对数生长期,10-5、10-6、10-7mol/L浓度的氢化可的松对活化小鼠T淋巴细胞增殖都有抑制作用,但各浓度组间无显著差异(P>0.05),而与对照组相比有显著差异(P<0.05)。[结论]在一定的浓度范围内氢化可的松对小鼠T淋巴细胞的增殖具有抑制作用,这为细胞移植免疫耐受的诱导提供了试验依据。[Objective] The aim was to investigate effects of Hydrocortisone on proliferation of mouse T lymphocytes.[Method] T lymphocytes separated aseptically from mouse spleen were stimulated in RPMI1640 complete medium plus Con A and transformed.The CD25 and CD69 on the surface of activated T cells as well as their co-expression rate were detected by Flow Cytometry（FCM）.The transformed T cells were divided into two groups： the control group cultured without Hydrocortisone and the experimental groups cultured with different concentration Hydrocortisone.The T cells were counted at different culture times and the growth curves were made.[Result] The CD25 and CD69 on T cells activated by Con A in three days and their co-expression rate reached the highest level,being 73.5%、58%as well as 32% respectively.The growth curves showed that both groups of T cells proliferation reached to exponential phase of growth cultured at the 2nd day with Hydrocortisone;the activated mouse T cells were inhibited by all of 10-5mol/L、10-6mol/L、10-7mol/L Hydrocortisone without significant difference（P0.05）among the three concentration groups,and with significant difference compared to the control group（P0.05）.[Conclusion] Hydrocortisone could inhibit proliferation of mouse T lymphocytes at a certain range of concentration,providing an experimental basis for animal model of cell transplantation in inducing immune tolerance.

关 键 词：氢化可的松 T细胞转化试验 CD25 CD69 

分 类 号：S85[农业科学—兽医学]