转bar基因水稻除草剂抗性遗传研究及其应用  

Inheritance of the Herbicide Resistance in Bar transgenic Rice and Its Application

作　　者：黎垣庆[1] 刘刚[2] 严文贵 许秋生[1] 

机构地区：[1]中国科学院华南植物研究所,广东广州510650 [2]中国科学院成都生物研究所,四川成都610041 [3]美国阿肯色严氏杂种优势与除草剂公司,美国司徒加ar72160

出　　处：《杂交水稻》2000年第1期40-43,共4页Hybrid Rice

摘　　要：试验用３ 个恢复系——测６４、明恢６３ 和特青作母本分别与３ 个抗除草剂转ｂａｒ基因水稻品种——Ｂｅｎ－ｇａｌ－Ｈｕ１０、Ｃｙｐｒｅｓｓ ＰＢ－６ 和Ｇｕｌｆｍ ｏｎｔ杂交，对其后代（Ｆ１，Ｆ２ 和ＢＣ１）进行遗传分析表明：除草剂的抗性是受一对显性核基因控制，通过杂交可将这对基因转育到恢复系，选配出抗除草剂杂交组合。应用这项技术可放宽对不育系完全雄性不育的严格要求，有利于优良不育系的培育及高产、优质、多抗新组合的选配。在秧田期喷施除草剂，可淘汰假杂种，确保杂种纯度。Three rice restoring lines (Minghui 63, Ce 64 and Teqing as female parents) were crossed with herbicide resistant bar transgenic rice cultivars (Cypress PB 6, Bengal Hu10 and Gulfmont as male parents). Genetic analysis on the generations of F 1, F 2 and BC 1 indicated that the resistance to herbicide Liberty was controlled by a single dominant nuclear gene. Therefore, the resistant gene to Liberty can be easily transferred to rice varieties. A breeding program has been proposed to transfer the resistant gene to a restoring line in hybrid rice system. The resistance to the herbicide can work as a marker to identify the true hybrid plants from selfing MS plants and other admixture plants by simply spraying the herbicide in seedling bed, thus the purity of hybrid rice can be ensured. With the aid of this technology, the requirement of complete sterility for male sterile lines may not be neccessary, which indicates that it will be beneficial not only for breeding new male sterile lines with fine grain quality, multi resistance, high outcrossing rate and good combining ability in both cytoplasmic male sterile system and environmentally induced male sterile system, but also for commercializing chemically induced male sterile system.

关 键 词：除草剂 抗除草剂基因 遗传 草丁膦 杂交水稻 

分 类 号：S511.053[农业科学—作物学] TQ457.29[化学工程—农药化工]