BTG1 as a New Candidate Gene for Muscle Growth in Pigs: Cloning,Expression and Association Analysis  

作　　者：Zheng Feng He Shen Zhiqiang Du Mengjin Zhu Bin Fan M. F. Rothschild Shuhong Zhao Changchun Li 

机构地区：[1]Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education ＆ Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China [2]Department of Animal Science, Center for Integrated Animal Genomics, Iowa State University, Ames, 50011, USA

出　　处：《Journal of Animal Science and Biotechnology》2011年第3期121-130,共10页畜牧与生物技术杂志（英文版）

基　　金：supported by the Outstanding Youth Foundation of NSFC (31025026);the Creative Team Project of Chinese Ministry of Education (IRT-0831);the National Natural Science Foundation of China (30800606 and 31072010);the Natural Science Foundation of Hubei Province (2010CDB10106)

摘　　要：BTG1 （B-cell Translocation Gene 1） , a member of the BTG / TOB （Transducer of ErbB-2） family of anti-proliferation factors,has been proven to have an unfavorable effect on muscle fiber growth in several species. The porcine BTG1 gene was cloned and its 5＇ flanking promoter region sequence, and characterized the expression patterns in different tissues of adult pigs and in fetal skeletal muscle at different developmental stages in two breeds. The tissue distribution pattern analyses revealed that the mRNA of porcine BTG1 was ubiquitously expressed in the six tissues of both Landrace and Tongcheng pigs. Real-time quantitative reverse transcriptase-PCR results showed that BTG1 mRNA expression levels were significantly different among the three fetal ages in Tongcheng pigs,while no significant differences were found among the three ages in Landrace pigs. Furthermore,the expression of BTG1 in Landrace pigs was significantly lower than in Tongcheng pigs at all three ages. The temporal expression profiles of the BTG1 gene in mouse myoblast C 2 C 12 cells were shown to be consistent with those of the myogenin gene. A single nucleotide polymorphism （SNP） ,g. 281C 〉 T,was identified in the 3＇UTR and allele frequencies were detected in seven pig breed populations. Significant associations were found between the g. 281C 〉 T polymorphism and growth and meat quality traits. Our results indicate that the porcine BTG1 gene could play a potential role in markerassisted selection and as such may be a gene of economic importance.BTG1 （B-cell Translocation Gene 1） , a member of the BTG / TOB （Transducer of ErbB-2） family of anti-proliferation factors,has been proven to have an unfavorable effect on muscle fiber growth in several species. The porcine BTG1 gene was cloned and its 5＇ flanking promoter region sequence, and characterized the expression patterns in different tissues of adult pigs and in fetal skeletal muscle at different developmental stages in two breeds. The tissue distribution pattern analyses revealed that the mRNA of porcine BTG1 was ubiquitously expressed in the six tissues of both Landrace and Tongcheng pigs. Real-time quantitative reverse transcriptase-PCR results showed that BTG1 mRNA expression levels were significantly different among the three fetal ages in Tongcheng pigs,while no significant differences were found among the three ages in Landrace pigs. Furthermore,the expression of BTG1 in Landrace pigs was significantly lower than in Tongcheng pigs at all three ages. The temporal expression profiles of the BTG1 gene in mouse myoblast C 2 C 12 cells were shown to be consistent with those of the myogenin gene. A single nucleotide polymorphism （SNP） ,g. 281C 〉 T,was identified in the 3＇UTR and allele frequencies were detected in seven pig breed populations. Significant associations were found between the g. 281C 〉 T polymorphism and growth and meat quality traits. Our results indicate that the porcine BTG1 gene could play a potential role in markerassisted selection and as such may be a gene of economic importance.

关 键 词：BTG1 C 2 C 12 cells gene expression muscle development PIGS 

分 类 号：Q78[生物学—分子生物学] S828.2[农业科学—畜牧学]