Maternal Administration of GHRP-6-loaded PLGA-PEG-PLGA Thermosensitive Hydrogel Increases Growth and Vitality of Piglets  

作　　者：Xiaohui Ren Zhunli Qi Xiuwen Zhang Jianwei Dai Linlin Hao Songcai Liu Yongliang Zhang 

机构地区：[1]College of Ocean, Hebei Agriculture University, Qinhuangdao, Hebei, 066003, China [2]Department of Biochemistry, Guangzhou Medical College, Guangzhou, Guangdong, 510182, China [3]Department of Biochemistry, Veterinary College, Jinlin University, Changchun, Jilin, 130062, China [4]College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, 510642, China

出　　处：《Journal of Animal Science and Biotechnology》2011年第3期173-178,共6页畜牧与生物技术杂志（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (u0731004, 30771572 );the National Basic Research Program of China (2009CB941601);the Hebei Province Nature Science Foundation (0722553)

摘　　要：Both growth hormone-releasing peptide 6 （GHRP-6） and growth hormone-releasing hormone （GHRH） have potent GH-releasing activity in animals. We have previously demonstrated that the administration of a plasmid encoding the GHRH gene to pregnant mice and pig augmented long-term growth in first generation progeny,and that the administration of GHRP-6 results in growth augmentation in mice and rabbits. However,it has not yet been reported if GHRP-6 induces intergenerational growth effects in pigs. Ploy lactic-co-glycolic acid （PLGA） microsphere adsorption of treatment proteins enhances gene expression,genetic immunization and the ability to protect plasmid DNA and peptides from degradation. The cur-rent study was conducted to determine the growth performance of piglets born to gilts treated with GHRP-6 incorporated into thermosensitive PLGA-PEG-PLGA triblock copolymers. Gilts were injected intra-muscularly once at day 85 of gestation with 30 mg of GHRP6-loaded thermosensitive PLGA-PEG-PLGA triblock copolymers. Piglets were weighed periodically between birth and 28 days. Mean body weights of piglets born to GHRP-6-treated gilts were 6. 58% to 18. 89% （P 〈 0. 05 ） greater than those of piglets born to control gilts. This study confirms that enhanced maternal GHRP-6 mediated by thermosensitive PLGA-PEG-PLGA can augment growth of piglets.Both growth hormone-releasing peptide 6 （GHRP-6） and growth hormone-releasing hormone （GHRH） have potent GH-releasing activity in animals. We have previously demonstrated that the administration of a plasmid encoding the GHRH gene to pregnant mice and pig augmented long-term growth in first generation progeny,and that the administration of GHRP-6 results in growth augmentation in mice and rabbits. However,it has not yet been reported if GHRP-6 induces intergenerational growth effects in pigs. Ploy lactic-co-glycolic acid （PLGA） microsphere adsorption of treatment proteins enhances gene expression,genetic immunization and the ability to protect plasmid DNA and peptides from degradation. The cur-rent study was conducted to determine the growth performance of piglets born to gilts treated with GHRP-6 incorporated into thermosensitive PLGA-PEG-PLGA triblock copolymers. Gilts were injected intra-muscularly once at day 85 of gestation with 30 mg of GHRP6-loaded thermosensitive PLGA-PEG-PLGA triblock copolymers. Piglets were weighed periodically between birth and 28 days. Mean body weights of piglets born to GHRP-6-treated gilts were 6. 58% to 18. 89% （P 〈 0. 05 ） greater than those of piglets born to control gilts. This study confirms that enhanced maternal GHRP-6 mediated by thermosensitive PLGA-PEG-PLGA can augment growth of piglets.

关 键 词：GH GHRP-6 growth performance PIG PLGA-PEG-PLGA 

分 类 号：S828.5[农业科学—畜牧学] Q575.11[农业科学—畜牧兽医]