Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice  

作　　者：YE ShuiFeng YU ShunWu SHU LieBo WU JinHong WU AiZhong LUO LiJun 

机构地区：[1]Shanghai Agrobiological Gene Center,Shanghai 201106,China [2]School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 201101,China [3]Shanghai Academy of Agricultural Sciences,Shanghai 201106,China

出　　处：《Chinese Science Bulletin》2012年第4期336-343,共8页

基　　金：supported by the National Program on Research and Development of Transgenic Plants of China(2008ZX08001-001);the Key Project of Shanghai Municipal Agriculture Commission(2009-6-4-2);the Shanghai Municipal Science and Technology Commission;Shanghai Postdoctoral Fund(10R21420400)

摘　　要：Plant heat shock proteins (Hsps) facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of rice cv. IRAT 109 leaf samples at the seedling stage under drought stress. To obtain additional information on the 9 OsHsp genes, this study focused on an expression profile analysis at different development stages throughout the life cycle of rice, and under different abiotic stresses and phyto-hormone treatments. The 9 genes exhibited distinctive expression patterns in different organs or development stages. Five of the genes (OsHsp72.90, OsHsp72.57, OsHsp71.18, OsHsp24.15 and OsHsp18.03) showed high expression in the endosperm, indicating that OsHsp genes may play important roles in rice seed development. All 9 OsHsps were up-regulated under heat, polyethylene glycol, and abscisic acid treatment, whereas salt stress caused up-regulation of 6 genes (OsHsp93.04, OsHsp71.10, OsHsp71.18, OsHsp72.57, OsHsp24.15 and OsHsp18.03) and cold stress resulted in down-regulation of OsHsp93.04 and OsHsp72.57. These diverse expression profiles imply potential functional diversity of the Hsp gene family in rice.Plant heat shock proteins （Hsps） facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of rice cv. IRAT 109 leaf samples at the seedling stage under drought stress. To obtain additional information on the 90sHsp genes, this study focused on an expression profile analysis at different development stages throughout the life cycle of rice, and under different abiotic stresses and phytohormone treatments. The 9 genes exhibited distinctive expression patterns in different organs or development stages. Five of the genes （OsHsp72.90, OsHsp72.57, OsHsp71.18, OsHsp24.15 and OsHspl8.03） showed high expression in the endosperm, indi- cating that OsHsp genes may play important roles in rice seed development. All 9 OsHsps were up-regulated under heat, polyethylene glycol, and abscisic acid treatment, whereas salt stress caused up-regulation of 6 genes （OsHsp93.04, OsHsp71.10, OsHsp71.18, OsHsp72.57, OsHsp24.15 and OsHspl8.03） and cold stress resulted in down-regulation of OsHsp93.04 and OsHsp72.57. These diverse expression profiles imply potential functional diversity of the Hsp gene family in rice.

关 键 词：表达谱分析 热休克蛋白 蛋白基因 水稻品种 生命周期 逆境 蛋白质折叠 蛋白质组分 

分 类 号：S852.65[农业科学—基础兽医学] Q785[农业科学—兽医学]