红外线照射对人表皮黑素细胞影响的研究  

Influences of infrared irradiation on human epidermal melanocytes

作　　者：张悦[1] 赵广[1] 孟如松[1] 宋文婷[1] 邵丽芳[1] 牛建荣[1] 

机构地区：[1]解放军空军总医院皮肤科,北京100142

出　　处：《中华皮肤科杂志》2012年第2期117-120,共4页Chinese Journal of Dermatology

摘　　要：目的 探讨红外线（infrared,JR）照射促进体外培养的正常人表皮黑素细胞的酪氨酸酶活性及黑素含量的作用,寻找IR照射的最佳剂量.方法 将体外培养的正常人表皮黑素细胞分别给予不同的红外线剂量：0 J/cm2（对照组）、20 J/cm2、60 J/cm2、80 J/cm2、100 J/cm2、140 J/cm2、240 J/cm2、320 J/cm2,采用四甲基偶氮唑蓝（MTT）法选择最佳剂量并以此作为照射条件,连续3d,检测黑素细胞的酪氨酸酶活性、酪氨酸酶活性增长率、黑素含量、红外线照射对黑素细胞影响率以及细胞周期.结果 红外线照射的最佳剂量为80 J/cm2,此照射下的酪氨酸酶活性0.3601±0.0301,高于对照组0.3114±0.0341 （P＜ 0.05）,酪氨酸酶活性增长率为15.64％.照射组的黑素细胞含量为0.2748±0.0243,高于对照组0.2325±0.0254（P＜0.05）,红外线照射对黑素细胞黑素含量影响率18.19％.细胞周期检测结果显示,G1细胞比例明显低于对照组（P＜0.01）,G2期和S期细胞比例较对照组增加（P＜0.05）.结论 80 J/cm2是IR照射的理想剂量,可以增加黑素细胞的黑素含量,提高酪氨酸酶活性,促进黑素细胞分化增殖.Objective To observe the increasing effect of infrared irradiation on tyrosinase activity and melanin content in cultured normal human epidermal melanocytes in vitro and to explore the optimal dose of infrared irradiation.Methods Epidermal melanocytes were isolated from normal human foreskin tissue,and subjected to primary culture.Methyl thiazolyl tetrazolium （MTT） assay was performed to evaluate the effect of different doses（0,20,60,80,100,140,240,320 J/cm2）of infrared light on the proliferation of melanocytes and to select the optimal irradiation dose.Then,melanocytes were irradiated with infrared light at the optimal dose for 3 consecutive days followed by the determination of tyrosinase activity,melanin content,and cell cycle via dopa oxidation assay,NaOH solubilization method and flow cytometry,respectively.Results The best intervention dose of infrared light was 80 J/cm2.The tyrosinase activity （A492 nm） and melanin content （A492 nm）were 0.3601 ± 0.0301 and 0.2748 ± 0.0243 respectively in melanocytes after irradiation with infrared light of 80 J/cm2 for 3 days,significantly higher than those in unirradiated melanocytes （0.3114 ± 0.0341,0.2325 ±0.0254,respectively,both P 〈 0.05）,with an increase rate of 15.64% and 18.19% respectively.Cell cycle analysis revealed a decline in cell percentage in G1 phase （P 〈 0.01 ） but a concomitant increase in cell percentage in G2 and S phase （both P 〈 0.05） in irradiated melanocytes compared with unirradiated melanocytes.Conclusions The optimal dose of infrared light is 80 J/cm2 for the irradiation of melanocytes,and this dose of infrared light can increase melanin content,tyrosinase activity,differentiation and proliferation of melanocytes.

关 键 词：白癜风 红外线 黑素细胞 细胞周期 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]