两头尖中的香豆素类成分及其生物活性研究  

Coumarins of Anemone raddeana Regel.and their biological activity

作　　者：任风芝[1] 陈书红[1] 郑智慧[1] 张雪霞[1] 李丽红[1] 董爱华[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司,微生物药物国家研究工程中心,河北石家庄050015

出　　处：《药学学报》2012年第2期206-209,共4页Acta Pharmaceutica Sinica

摘　　要：为了研究两头尖的香豆素类化学成分,利用硅胶柱色谱及高效液相色谱等方法对两头尖提取物进行分离和纯化,根据理化性质和光谱数据鉴定化合物结构。从中分离得到两个新的香豆素类化合物,分别鉴定为4,7-二甲氧基-5-甲基-6-羟基香豆素(1)和4,7-二甲氧基-5-醛基-6-羟基香豆素(2)。生物活性试验显示两个化合物对肿瘤细胞的增殖有抑制作用,对维甲酸α受体(RARα)有激动活性,同时对人白细胞弹性蛋白酶也有抑制作用。To study the coumarins of Anemone raddeana Regel, the compounds were separated by silica gel column chromatography and HPLC. Their structures were identified by their physicochemical property and spectral analysis. Two new compounds were isolated and identified as 4, 7-dimethoxyl-5-methyl-6-hydroxy coumarin （1） and 4, 7-dimethoxyl-5-formyl-6-hydroxycoumarin （2）. The bioassays indicated that compounds 1 and 2 could significantly inhibit the proliferation of cancer cell, and showed the agonist effect on the transactivity ofretinoic acid receptor-a （RARa）. In addition, the two compounds had inhibitory effect against human leukocyte elastase （HLE）.

关 键 词：两头尖 香豆素 抗肿瘤活性 维甲酸受体 人白细胞弹性蛋白酶 

分 类 号：R284.1[医药卫生—中药学]