基于HMGR、SQS1、β-AS基因CNVs的甘草道地性机制研究  

Mechanism of genuineness of liquorice Glycyrrhiza uralensis based on CNVs of HMGR,SQS1 and β-AS gene

作　　者：刘颖[1] 刘东吉[1] 刘春生[1] 廖彩丽[1] 程小丽[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《药学学报》2012年第2期250-255,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30672615)

摘　　要：本文利用real-time PCR方法对不同产地甘草的3-羟基-3-甲基戊二酰CoA还原酶(3-hydroxy-3-methylglutaryl-CoA reductase,HMGR)、鲨稀合成酶1(squalene synthetase 1,SQS1)、β-香树脂醇合成酶(β-amyrin synthase,β-AS)基因的拷贝数进行了研究,发现不同产地的HMGR基因存在1～3个拷贝数变异,SQS1基因存在1～2个拷贝数变异,未发现β-AS基因拷贝数变异。甘草HMGR、SQS1、β-AS基因拷贝数存在5种自然组合类型:A型(2+1+1)、B型(1+1+1)、C型(3+2+1)、D型(2+2+1)和E型(3+1+1),其中内蒙古杭锦旗甘草存在A、B两种类型,A与B的比例为1∶1.3;内蒙古赤峰甘草也存在A、B两种类型,但A与B比例为3∶1;宁夏盐池甘草存在A、B、C、D 4种类型,A/B/C/D比例为1∶5.1∶1∶2,A/B比例为1∶5.1;甘肃民勤甘草存在A、B、E 3种类型,A/B/E比例为4.1∶2.1∶1,A/B比例为2∶1。本研究证明中药功能基因基因组拷贝数变异(copy number variations,CNVs)与产地具有相关性,可能是道地药材形成的机制之一。This study is to reveal the correlation between CNVs of HMGR, SQS 1, β-AS gene and genuineness of liquorice. Real-time PCR was used to detect the copy number of HMGR, SQS1, β-AS gene of liquorice. According to the results, the range of the copy number variation of HMGR gene was between 1 and 3, the copy number of SQS 1 gene was 1 or 2, and the copy number of β-AS gene was only 1. On the basis of the copy number of HMGR, SQS1 and β-AS gene, there were five groups, type A （2＋1＋1）, type B （1＋1＋1）, type C （3＋2＋1）, type D （2＋2＋1） and type E （3＋1＋1）. There were two types, type A and type B, in Hangjinqi of Inner Mongolia, and the ratio of A to B was 1 ： 1.3. There were also two types, type A and type B, in Chifeng of Inner Mongolia, and the ratio of A to B was 3 ： 1. There were four types, type A, type B, type C and type D, in Yanchi of Ningxia province, and the ratio of A to B was 1 ： 5.1. There were three types, type A, type B and type E, in Minqin of Gansu province, and the ratio of A to B was 2 ： 1. So CNVs mainly existed in the liquorice from Ningxia and Gansu provinces. While the genetic background of liquorice from Hangjinqi of Inner Mongolia was stabilized. The results of the experiment proved that the correlation between CNVs and origins was one of the reasons of genuineness of liquorice.

关 键 词：HMGR SQS1 β-AS CNVs 道地性 甘草 

分 类 号：R931[医药卫生—生药学]