几种经济作物根际拮抗细菌的多样性  

Diversity of antagonistic bacteria isolated from rhizosphere of several cash crops

作　　者：徐莹莹[1] 杜秉海[1,2] 姚良同[1] 靳奉理[1] 王翠翠[1] 王璇[1] 丁延芹[1] 

机构地区：[1]山东农业大学生命科学学院微生物学系,山东泰安271018 [2]山东省农业微生物重点实验室,山东泰安271018

出　　处：《应用生态学报》2012年第2期511-518,共8页Chinese Journal of Applied Ecology

基　　金：国家公益性行业(农业)科研专项(200903018;nyhyzx07-024)资助

摘　　要：采用抗菌谱测定以及BOXAIR-PCR、生理生化特征和16S rDNA序列分析等方法对分离自10种作物根际的55株拮抗细菌的多样性及主要拮抗菌类群进行了分析.结果表明:根际拮抗细菌的拮抗作用具有丰富的多样性;BOXAIR-PCR分析中供试菌在72.1%相似性水平上可以聚为7个群,85.0%相似性水平上聚为25个群;所有供试根际拮抗菌分别属于芽孢杆菌属(Bacillus)芽孢杆菌属(Paenibacillus)、短芽孢杆菌属(Brevibacillus)、假单胞菌属(Pseudomonas)和产碱菌属(Alcaligenes).作物根际拮抗菌具有丰富的遗传多样性和拮抗性能多样性.By adopting antimicrobial spectrum test,BOXAIR-PCR,physiological and biochemical,and 16S rDNA sequencing analysis,this paper analyzed the diversity of 55 antagonistic bacterial strains isolated from the rhizosphere of 10 cash crops.There was a high diversity of the antagonism of the strains.Based on BOXAIR-PCR,all the strains were clustered into 7 groups at the similarity level of 72.1%,and divided into 25 groups at the similarity level of 85.0%.All the strains belonged to Bacillus,Paenibacillus,Brevibacillus,Pseudomonas and Alcaligenes,respectively.The antagonistic bacteria isolated from the rhizosphere had high genetic diversity and high diversity in antagonistic activity.

关 键 词：根际 抗菌谱 BOXAIR-PCR 16S RDNA 多样性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]