High genetic diversity in population of Lepturichthys fimbriata from the Yangtze River revealed by microsatellite DNA analysis  

作　　者：ZHANG FuTie DUAN YouJian CAO ShanMao WANG JianWei TAN DeQing 

机构地区：[1]Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [2]Dalian Ocean University,Dalian 116023,China

出　　处：《Chinese Science Bulletin》2012年第5期487-491,共5页

基　　金：supported by joint funds of the National Natural Science Foundation of China and Yalong River Hydropower Development Foundation (50679095);China Yangtze Three Gorges Project Development Corporation (0714086);Non-profit Industry Financial Program of MWR of China (200901011)

摘　　要：Lepturichthys fimbriata (Günther) is one of the benthic and rock-attached fish species that is typically found in torrential flows of the upper reaches of the Yangtze River in China. Several dams in the Yangtze River (the Ertan Dam, the Three Gorges Dam, the Gezhouba Dam, the Xiluodu Dam and the Xiangjiaba Dam (the latter two dams are under construction)) may have significant effects on the habitat and spawning behaviors of L. fimbriata, and could ultimately threaten the survival of this fish. We studied the population genetic diversity of L. fimbriata samples collected at three sites within the Yangtze River and one of its tributaries, the Yalong River. Genetic diversity patterns were determined by analyzing genetic data from 14 polymorphic microsatellite loci isolated in Jinshaia sinensis (Sauvage et Dabry). A high genetic diversity among these L. fimbriata local populations was indicat- ed by the number of microsatellite alleles (A) and the expected heterozygosity. No reductions of genetic diversity in any L. fimbriata population were observed. However, significant population differentiations were observed among three local populations by pairwise comparisons (P<0.001). We deduced that L. fimbriata local populations were not small ones. In addition, the habitat behaviors of rock-attachment and possible residence of L. fimbriata could account for the genetic differences found in local populations.Lepturichthysfimbriata （Gunther） is one of the benthic and rock-attached fish species that is typically found in torrential flows of the upper reaches of the Yangtze River in China. Several dams in the Yangtze River （the Ertan Dam, the Three Gorges Dam, the Gezhouba Dam, the Xiluodu Dam and the Xiangjiaba Dam （the latter two dams are under construction）） may have significant effects on the habitat and spawning behaviors of L. fimbriata, and could ultimately threaten the survival of this fish. We studied the population genetic diversity of L. fimbriata samples collected at three sites within the Yangtze River and one of its tributaries, the Yalong River. Genetic diversity patterns were determined by analyzing genetic data from 14 polymorphic rnicrosatellite loci isolated in Jinshaia sinensis （Sanvage et Dabry）. A high genetic diversity among these L. fimbriata local populations was indicat- ed by the number of microsatellite alleles （A） and the expected heterozygosity. No reductions of genetic diversity in any L. tim- briata population were observed. However, significant population differentiations were observed among three local populations by pail＇wise comparisons （P〈0.001）. We deduced that L fimbriata local populations were not small ones. In addition, the habitat behaviors of rock-attachment and possible residence of L. fimbriata could account for the genetic differences found in local popu- lations.

关 键 词：种群遗传多样性 长江上游 黑斑病 人口观 DNA分析 微卫星 三峡大坝 显示 

分 类 号：S157[农业科学—土壤学] Q959.837[农业科学—农业基础科学]