硝酸银硅胶柱层析分离血浆不饱和脂肪酸  

Extraction and Separation of Unsaturated Fatty Acids in Blood Plasma by Silver Nitrate-silica Gel Column Chromatography

作　　者：成琪[1] 吕世明[1] 李昭华[2] 董先智[2] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]中国科学院生物物理研究所,北京100101

出　　处：《中国生物工程杂志》2012年第1期42-48,共7页China Biotechnology

摘　　要：目的:利用硝酸银硅胶填料有效分离出血浆混合脂肪酸中的亚油酸。方法:通过改进的FOLCH法提取血浆总脂后,再采用皂化、酸化水解的方法将总脂转化为混合脂肪酸。用ghosh法将硅胶改性为硝酸银硅胶后,以亚油酸为对象,通过静态吸附试验了解硝酸银硅胶对不饱和脂肪酸的吸附特性,采用柱层析的方法分离血浆混合脂肪酸中的亚油酸。结果:血浆与有机溶剂在1∶5时既能有效萃取血浆总脂,用正己烷∶二氯甲烷∶乙醚=89∶10∶1作为洗脱剂,将洗脱液甲酯化后进行GC和GC-MS检测,硝酸银硅胶柱的洗脱液中亚油酸的纯度60.74%,硅胶柱的为23.65%,不饱和脂肪酸得到了较好的纯化。A ripid and efficient method for separation of unsaturated fatty acid is described as the FOLCH method that is extract total lipids of blood plasma with 1∶20 blood plasma to extrcatant（methanol∶chloroform=1∶2）.The method is modification from it that allows the proption of blood plasma to extrcatant（methanol∶dichloromethane=1∶2）reducing from1∶20 to 1∶5.we prepared silver nitrate-silica gel by the Ghosh method,and,drew the adsorption isotherm of linoleic acid to understand the character of silver nitrate-silica gel for adsorbing unsaturated fatty acid.In addition,fatty acids were eluted with a mixture of n-hexane∶dichloromethane∶ethyl ether=89∶10∶1 after the sample has been loaded column.After that,linoleic acid was quantitatively analysed and the others fatty acids were qualitatively analysed by GC and GC-MS.There are distinct differences for purification of linoleic acid and the others fatty acids in the eluent,the silver nitrate-silica gel can well purificated unsaturated fatty acids in blood plasma.

关 键 词：硝酸银硅胶 血浆 亚油酸 不饱和脂肪酸 

分 类 号：Q5[生物学—生物化学]