芪苈强心胶囊对H_2O_2诱导的H9C2大鼠心肌细胞PPARα表达的影响  

Effects of Qiliqiangxin capsule on the expression of PPARα of H9C2 cell induced by H_2O_2

作　　者：徐涛[1] 郭丽峰[1] 任峻青[1] 李方江[1] 陈立锋[1] 

机构地区：[1]河北北方学院附属第一医院心内科,河北省张家口市075000

出　　处：《河北医药》2012年第3期325-327,共3页Hebei Medical Journal

基　　金：河北省张家口市科技局科技研究与发展计划项目(编号:0921070D)

摘　　要：目的观察芪苈强心胶囊对H9C2大鼠心肌细胞PPARα表达的影响,探讨芪苈强心胶囊抗心力衰竭作用的相关机制。方法 30只H9C2大鼠心肌细胞分为正常对照组、模型组、芪苈强心组、WY14643组、芪苈强心组+WY14643组,每组6只,采用100μmol/LH2O2处理6h造成心肌细胞损伤模型,5组给药后继续培养12、24、48h,测定细胞增殖率和LDH漏出率。5组给药孵育48h后,收集细胞,RT-PCR和Westernblot法检测PPARαmRNA和蛋白的表达。结果与模型组比较,芪苈强心组、WY14643组、芪苈强心组+WY14643组均能增加H9C2细胞增殖活性(P<0.05或<0.01),抑制LDH漏出率(P<0.05或<0.01),RT-RCR和Westernblot方法结果显示芪苈强心组、WY14643组、芪苈强心组+WY14643组PPARαmRNA和蛋白表达显著上升(P<0.05或<0.01)。结论芪苈强心胶囊可通过上调PPARα的表达,发挥其心肌细胞的保护作用。Objective To investigate the effects of Qiliqiangxin capsule on the expression of PPARct of H9C2 cell induced by H2O2, and to explore the possible action mechanisms of Qiliqiangxin capsule in resisting heart failure. Methods The H9C2 cells from 30 rats were divided into the normal control group, model group, Qiliqiangxin group,WY14643 group, Qiliqiangxin ＋ WY14643 group ,with 6 rats in each group. The animal models myocardial cell injury were established by treating the rats with 100μmol/L H2O2 for 6h. After administration for the 5 groups, the cells were cultured continuously for 12h ,24h,48h respectively. The rates of cell proliferation and LDH leakage were detected. The cells in 5 groups were collected after 48h, the expressions of PPARα mRNA and protein were detected by RT-PCR and Western Blot. Results As compared with model group, Qiliqiangxin group, WY14643 group and Qiliqiangxin ＋ WY14643 group could increase the activity of cell proliferation （ P 〈0.05 or 〈0.01 ） ,and could decrease LDH leakage rate （ P 〈0.05 or 〈0.01 ）. The results of RT-RCR and Western Blot detection showed that the latter 3 groups could significantly up-regulate the expression of PPARα mRNA and protein （ P 〈 0.05 or 〈 0. 01 ）. Conclusion Qiliqiangxinjiaonang can protect myocardial cells （ H9C2 ） by up-regulating the expression of PPARα.

关 键 词：芪苈强心胶囊 H9C2细胞 PPARΑ 大鼠 

分 类 号：R331.31[医药卫生—人体生理学]