自拟中药对人工感染IBV雏鸡免疫器官及血清IgG的影响  

Effects of Chinese herbal compound prescription on immune organ index and IgG level in chicken challenged by IBV-M41

作　　者：陈炅然[1] 严作廷[1] 王萌[2] 王东升[1] 张世栋[1] 叶得河[2] 王玲[1] 于远光[2] 魏兴军 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室,甘肃兰州730050 [2]甘肃农业大学动物医学院 [3]兰州华陇家禽育种有限公司

出　　处：《中兽医医药杂志》2012年第1期35-40,共6页Journal of Traditional Chinese Veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(BRF090403)

摘　　要：目的:研究自拟中药方剂对鸡传染性支气管炎的防治效果。方法:首先选取SPF鸡胚测定IBV-M41的ELD50,以确定攻毒剂量。然后将400只19日龄无IB抗体的健康雏鸡随机分为8组,每组50只,分别为预防高(2.0 mL/次)、中(1.0 mL/次)、低(0.5 mL/次)组,治疗高(4.0 mL/次)、中(2.0 mL/次)、低(1.0 mL/次)组,阳性对照组和阴性对照组,19日龄时预防组开始用药,连用5 d。21日龄时除阴性对照组外其余各组均以100 ELD50剂量的IBV-M41人工感染雏鸡。待症状出现24 h后,治疗组开始给药,连用5 d,期间进行临床观察。考察该自拟中药的临床预防及治疗效果,并测定不同处理组不同日龄患病雏鸡免疫器官指数及血清IgG的质量浓度。结果:在用药过程中,自拟复方中药的预防和治疗不同剂量组免疫器官指数与阴性对照组相比差异显著,组间差异不显著;用药疗程结束后,各组间差异不显著。但该中药方剂对不同日龄不同处理组雏鸡血清IgG的质量浓度影响明显,且与阴性、阳性对照组相比差异极显著。结论:该中药方剂对雏鸡法氏囊及脾脏发育有促进作用,且诱生IgG,使其血清含量显著提高,对机体产生免疫保护,从而达到对鸡传染性支气管炎的防治效果。Objective： The study was carried out to investigate the effect of self-made Chinese herbal medicine on immune organ index and IgG level in serum during prevention and treatment of fowl infectious bronchitis in chicken. Methods： Four hundred 19-day-old chickens with IB antibody free were divided into eight groups such as follows： 3 groups treated with high （2.0 mL/time）, middle （ 1.0 mL/ time） and low （0.5 mL/time） dose of the herhal solution for prevent＇.＇ve trial, another 3 groups treated with high （4.0 mL/time）, middle （2.0 mL/time） and low （ 1.0 mldtime） dose of the herbal solution for treatment trial, positive control group and negative control group, 50 in each group. The preventive groups were treated with herbal solution on day 19 for 5 days. All the groups were challenged by IBV- M41 on clay 21 except the negative control group. The treatment groups underwent herbal solution for 5 d after the clinic symptom coming. Recorded the clinic observed changes during the whole experiment. Immune organ index and the activities of IgG in serum in chicken from all groups were measured and analyzed at different time. Results： During the course of tl^atment, compared with negative and positive group, the treatment and preventive groups had extremely significant difference in immune index, while the difference was not significant between different dose groups. After the course of treatment, the immune index of all groups had no significant difference. Refer to the concentration of IgG in serum, the treatment and preventive groups had extremely significant compared with negative and positive group. Conclusion： The self-made Chinese herbal compound can impose a positive action on prevention and treatment of fbwl infectious bronchitis in chicken by inducing IgG persistently and improving the immune organs healthy development, so that it can adjust the immunity function to achieve the aim of antiviral action.

关 键 词：鸡传染性支气管炎 自拟中药方剂 法氏囊指数 脾脏指数 IgG 

分 类 号：S852.43[农业科学—基础兽医学]