转ipt基因四季海棠植株抗旱性分析  

Analysis on Drough Tolerance of Transgenic Begonia semperflorens Plant with ipt Gene

作　　者：张雷[1] 鲍平秋[1] 

机构地区：[1]北京联合大学特殊教育学院,北京100075

出　　处：《湖北农业科学》2012年第1期83-87,共5页Hubei Agricultural Sciences

基　　金：北京市教育委员会科技发展计划项目(KM200511417006)

摘　　要：利用ipt重组表达载体质粒,通过农杆菌介导法建立了遗传转化体系,并筛选出四季海棠(Begonia semperflorens)转ipt基因植株。研究结果发现,在细胞分裂素的调控下,转基因植株的高度、叶片面积均有不同程度的减小,但根系长度增加;在渗透胁迫条件下,转基因植株体内脯氨酸含量和SOD活性都明显增加。通过温室栽种,发现在同等情况下转基因植株的耐旱性明显强于非转基因植株。Ipt（isopentenyl transferase） is a key enzyme responsing to the biosynthesis of cytokinin.The transgenic plants with ipt gene were obtained by Agrobacterium-mediated genetic transformation system of Begonia.Compared to non-transgenic plants,the height,leaf area and leaf number of transgenic plants decreased in different degrees.However,the length of the roots increased.The antioxidant enzyme（SOD） activities and the content of proline were increased significantly in transgenic plant under osmotic stress conditions induced by polyethylene glycol（PEG） 6000.The drought tolerance of transgenic plant was stronger than that of non-genetically modified plants in greenhouse.

关 键 词：四季海棠(Begonia semperflorens) 异戊烯基转移酶基因(ipt) 细胞分裂素 遗传转化体系 抗旱性 

分 类 号：S661.4[农业科学—果树学] Q945.78[农业科学—园艺学]